Glycoside Hydrolase Family 13 / Subf 32

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (425); Uniprot accession (76); PDB accession (9); 3D entries (1); cryst (0)
| 1 | 2 | 3 | 4 |
Bacteria
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 SIRAN7128   Streptomyces iranensis CDR10953.1     32
 SLA_1809   Streptomyces laurentii ATCC 31255 BAU82747.1     32
 SLAV_26100 (AmL)   Streptomyces lavendulae subsp. lavendulae CCM 3239 ATZ27012.1     32
 Sle_49310   Streptomyces leeuwenhoekii C34 (= DSM 42122 = NRRL B-24963) CQR64389.1     32
 α-amylase (Aml) 3.2.1.1 Streptomyces limosus ATCC 19778 AAA88554.1 P09794   32
 SLINC_2556   Streptomyces lincolnensis NRRL 2936 ANS64780.1     32
 α-amylase (AmyA)   Streptomyces lividans ATCC 19844 ABZ81609.1 B0ZCN0   32
 α-amylase (Aml;AmlA) 3.2.1.1 Streptomyces lividans TK21 CAA73926.1 O86876   32
 α-amylase (AmlB) 3.2.1.1 Streptomyces lividans TK24 CAB06622.1 P97179   32
 SLIV_04145 (AmL)   Streptomyces lividans TK24 AIJ11852.1     32
 amylase (fragment)   Streptomyces lonarensis ADZ45287.1     32
 amylase   Streptomyces lonarensis NCL 716 ADZ45287.2     32
 SL103_22760   Streptomyces lydicus 103 AOP48684.1     32
 SMALA_2143   Streptomyces malaysiensis DSM 4137 ATL82377.1     32
 BBN63_25315   Streptomyces niveus SCSIO 3406 AQU69006.1     32
 SNOD_30550   Streptomyces nodosus ATCC 14899 AJE43854.1     32
 SNOUR_08095   Streptomyces noursei ATCC 11455 ANZ14938.1     32
 B1H29_04330   Streptomyces pactum ACT12 AQS66264.1     32
 B1H29_26265   Streptomyces pactum ACT12 AQS69916.1     32
 BC342_08765   Streptomyces pactum KLBMP 5084 AOW86628.1     32
 Spa2297_09300   Streptomyces parvulus 2297 ANJ07185.1     32
 CGZ69_28340   Streptomyces peucetius subsp. caesius ATCC 27952 ATW51177.1     32
 LK06_008085   Streptomyces pluripotens MUSC 135 ARP69930.1     32
 LK07_09185   Streptomyces pluripotens MUSC 137 ASN24185.1     32
 Sfla_4612   Streptomyces pratensis ATCC 33331 ADW06013.1 C9NEV1   32
 SPRI_1863   Streptomyces pristinaespiralis HCCB 10218 ALC20169.1     32
 BFF78_30055   Streptomyces puniciscabiei TW1S1 AOR37415.1     32
 M271_35280   Streptomyces rapamycinicus NRRL 5491 AGP58463.1     32
 TUE45_06959 (Aml_3)   Streptomyces reticuli CUW32210.1     32
 TUE45_02766 (Aml_1)   Streptomyces reticuli CUW28036.1     32
 A4G23_01392 (Aml_1)   Streptomyces rubrolavendulae MJM4426 AOT58579.1     32
 A4G23_04447 (Aml_2)   Streptomyces rubrolavendulae MJM4426 AOT61559.1     32
 SCAB_66681 (AmlB)   Streptomyces scabiei 87.22 CBG73668.1 C9ZHE6   32
 A7J05_03405   Streptomyces silaceus ACCC40021 APY84923.1     32
 A7J05_03520   Streptomyces silaceus ACCC40021 APY84940.1     32
 unnamed protein product   Streptomyces sp. CAD82886.1     32
 unnamed protein product   Streptomyces sp. CAD82885.1     32
 CGL27_14930   Streptomyces sp. 11-1-2 ASQ94196.1     32
 SAMN05428941_6974   Streptomyces sp. 2114.2 SDT80227.1     32
 SAMN06272775_0889   Streptomyces sp. 2323.1 SOE09822.1     32
 SAMN05444521_6486   Streptomyces sp. 3124.6 SHI26530.1     32
 ASR50_10825   Streptomyces sp. 4F ALV49862.1     32
 GZL_01813   Streptomyces sp. 769 AJC54409.1     32
 T261_1023   Streptomyces sp. A02 AJT62711.3
AJT62711.1
AJT62711.2
    32
 NI25_04090   Streptomyces sp. CCM_MD2014 AIV32806.1     32
 AS200_31700   Streptomyces sp. CdTB01 ALV38889.1     32
 AS200_31705   Streptomyces sp. CdTB01 ALV36102.1     32
 ABE83_26925   Streptomyces sp. CFMR 7 ALC30277.1     32
 CXR04_06945   Streptomyces sp. CMB-StM0423 AUH40018.1     32
 AA958_08825   Streptomyces sp. CNQ-509 AKH82314.1     32
 α-amylase   Streptomyces sp. E-2248 BAF49593.1 A4GZ20   32
 B1K54_11400   Streptomyces sp. fd1-xmd AQT72204.1     32
 SFR_5457   Streptomyces sp. FR-008 ALM42072.1     32
 maltogenic α-amylase   Streptomyces sp. GXT6 ADB89947.1 D3K0N7   32
 CFP59_06493 (AmL)   Streptomyces sp. M56 AUA14315.1     32
 M444_11355   Streptomyces sp. Mg1 AKL65907.1     32
 STRMOE7_32770   Streptomyces sp. MOE7 ARH94279.1     32
 F750_2074   Streptomyces sp. PAMC 26508 AGJ54569.1     32
 QR97_03070   Streptomyces sp. PBH53 AKN68929.1     32
 QR97_39515   Streptomyces sp. PBH53 AKN74972.1     32
 A4E84_11515   Streptomyces sp. S10(2016) AMW10089.1     32
 A6E92_24020   Streptomyces sp. S8 ARI54891.1     32
 A8713_034740 (fragment)   Streptomyces sp. SAT1 ANO42416.1     32
 ORF   Streptomyces sp. SCSIO 03032 AIA26431.1
ARQ72566.1
    32
 B6R96_24915   Streptomyces sp. Sge12 ARE76782.1     32
 SACTE_1640   Streptomyces sp. SirexAA-E AEN09556.1     32
 SACTE_5685   Streptomyces sp. SirexAA-E AEN13474.1     32
 SAMN05216371_5499   Streptomyces sp. TLI_053 SDT77652.1     32
 BSL84_09835   Streptomyces sp. TN58 APU40027.1     32
 α-amylase 3.2.1.1 Streptomyces sp. TO1 CAA73775.1 O65947   32
 TK78_25885   Streptomyces sp. Tue 6075 Tue6075 APS21971.1     32
 α-amylase (Amy) 3.2.1.1 Streptomyces sp. WL6 AAA82875.1 Q59964   32
 α-amylase 3.2.1.1 Streptomyces thermoviolaceus CUB74 AAA26697.1 P27350   32
 SVEN_1889   Streptomyces venezuelae ATCC 10712 CCA55176.1     32
 α-amylase (Aml) 3.2.1.1 Streptomyces venezuelae ATCC 15068 AAB36561.1 P22998   32
 BN2537_12585   Streptomyces venezuelae ATCC 15439 CUM41810.1     32
 AQF52_2259   Streptomyces venezuelae ATCC 15439 ALO07854.1     32
 SVTN_10835   Streptomyces vietnamensis GIM4.0001 AJF64853.1     32
 B1H20_07980   Streptomyces violaceoruber S21 ARF61357.1     32
 Strvi_7302   Streptomyces violaceusniger Tu 4113 AEM86663.1     32
 SXIM_11390   Streptomyces xiamenensis 318 AKG42523.1     32
 Sros_3337   Streptosporangium roseum DSM 43021 ACZ86276.1 D2BDI7   32
 Sros_4292   Streptosporangium roseum DSM 43021 ACZ87182.1 D2AZU8   32
 RPIT_12550   Tessaracoccus flavus RP1T AQP45531.1     32
 BKM78_09375   Tessaracoccus sp. T2.5-30 AQX16098.1     32
 maltotriose-producing α-amylase (Tfa;Amy13) 3.2.1.1
3.2.1.116
Thermobifida fusca NTU22 ABF13430.1 Q1KLC8   32
 α-amylase (TFA;Tfu_0985)   Thermobifida fusca YX AAZ55023.1 Q47R94   32
 α-amylase (Tam) 3.2.1.1 Thermomonospora curvata CAA41881.1 P29750   32
 THII_0266   Thioploca ingrica BAP54563.1     32
 Alpha amylase, C-terminal all-beta domain, partial (fragment)   uncultured bacterium APO30162.1     32
 Alpha amylase, catalytic domain (fragment)   uncultured bacterium APO30161.1     32
 VAB18032_27916   Verrucosispora maris AB-18-032 AEB46656.1     32
 VAB18032_27951   Verrucosispora maris AB-18-032 AEB46663.1     32
 JV59_24880   Vibrio coralliilyticus OCN014 AIS58275.1     32
 IX92_27920   Vibrio coralliilyticus RE98 AIW22870.1     32
 AL536_16095   Vibrio fluvialis ATCC 33809 AMF94961.1     32
 BSQ33_18910   Vibrio gazogenes ATCC 43942 ASA57804.1     32
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