Glycoside Hydrolase Family 13 / Subf 32

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (393); Uniprot accession (76); PDB accession (9); 3D entries (1); cryst (0)
| 1 | 2 | 3 | 4 |
Bacteria
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 S4054_10060   Pseudoalteromonas luteoviolacea S4054 AOT17995.1     32
 S40542_10065   Pseudoalteromonas luteoviolacea S40542 AOT13083.1     32
 S40542_10060   Pseudoalteromonas luteoviolacea S40542 AOT13082.1     32
 S4054249_10065   Pseudoalteromonas luteoviolacea S4054249 AOT08166.1     32
 S4054249_10060   Pseudoalteromonas luteoviolacea S4054249 AOT08165.1     32
 OM33_20925   Pseudoalteromonas piratica OCN003 AIY67486.1     32
 B1L02_23035   Pseudoalteromonas piscicida DE2-B ASD69722.1     32
 PPIS_b0635 (AmyA)   Pseudoalteromonas piscicida JCM 20779 ATD09756.1     32
 AOR04_14490   Pseudoalteromonas sp. 1_2015MBL_MicDiv ATG78638.1     32
 D172_002960   Pseudoalteromonas sp. Bsw20308 ALQ07114.1     32
 α-amylase   Pseudoalteromonas sp. GS230 ACJ03926.1 B6SED8   32
 α-amylase   Pseudoalteromonas sp. MY-3 ACF40218.1 C0J0Y3   32
 PTUN_a2361 (AmyA)   Pseudoalteromonas tunicata D2 ATC94851.1     32
 α-amylase 2 (Amy2) 3.2.1.1 Pseudomonas sp. KFCC10818 AAA86835.1 Q52413   32
 α-amylase 4 (Amy4) 3.2.1.1 Pseudomonas sp. KFCC10818 AAK17994.1 Q9AGG4   32
 PjaA   Pseudomonas sp. W7 AAF82611.1 Q9KK55   32
 N018_10220   Pseudomonas syringae CC1557 AHG43543.1     32
 CPZ87_22285   Rhizobacter gummiphilus NBRC 109400 ATU67083.1     32
 CPZ87_22285   Rhizobacter gummiphilus NBRC 109400 ATU67083.1     32
 A4W93_22155   Rhizobacter gummiphilus NS21 ARN22391.1     32
 AWN76_016255   Rhodothermaceae bacterium RA ARA94555.1     32
 RD2015_699   Roseateles depolymerans KCTC 42856 ALV05195.1     32
 Svir_05290   Saccharomonospora viridis DSM 43017 ACU95603.1 C7MUP0   32
 Sde_2938 (Amy13D)   Saccharophagus degradans 2-40 ABD82195.1 Q21GI4   32
 maltotetraose-forming amylase   Saccharophagus degradans STB03 AIV43244.1     32
 SACE_1658   Saccharopolyspora erythraea NRRL 2338 CAM00977.1 A4FAA2   32
 Sare_2896   Salinispora arenicola CNS-205 ABV98722.1 A8M6K2   32
 Strop_2001   Salinispora tropica CNB-440 ABP54455.1 A4X6F5   32
 Strop_2686   Salinispora tropica CNB-440 ABP55130.1 A4X8D0   32
 SCIP_0232   Scardovia inopinata JCM 12537 BAR06299.1     32
 M5M_08225   Simiduia agarivorans SA1 = DSM 21679 AFU98834.1     32
 Snas_1247   Stackebrandtia nassauensis DSM 44728 ADD40957.1 D3PU11   32
 STAUR_7765   Stigmatella aurantiaca DW4/3-1 ADO75520.1 Q08XX6   32
 SMD11_4846   Streptomyces albireticuli SMD11 ARZ70439.1     32
 SMD44_01121   Streptomyces alboflavus MDJK44 ARX81723.1     32
 DC74_6944   Streptomyces albulus NK660 AIA07374.1     32
 SAZ_36050   Streptomyces albulus ZPM AKA07210.1     32
 α-amylase 3.2.1.1 Streptomyces albus AAA96317.1 Q53633   32
 SLNHY_0493   Streptomyces albus BK3-25 AOU75184.1     32
 SLNWT_0495   Streptomyces albus DSM 41398 AJE80871.1     32
 XNR_4649   Streptomyces albus J1074 AGI90971.1     32
 Salbus254_4913   Streptomyces albus SM254 AMM11366.1     32
 SAM23877_2313 (AmY)   Streptomyces ambofaciens ATCC 23877 AKZ55362.1     32
 SAM40697_2114   Streptomyces ambofaciens DSM 40697 ANB06074.1     32
 BV401_14185   Streptomyces autolyticus CGMCC0516 AQA11464.1     32
 α-amylase (SAV5981) 3.2.1.1 Streptomyces avermitilis MA-4680 = NBRC 14893 BAC73693.1
NP_827158.1
    32
 SBI_07736 (Amya2)   Streptomyces bingchenggensis BCW-1 ADI10856.1 D7CD09   32
 SCAT_0273 (AmL)   Streptomyces cattleya NRRL 8057 = DSM 46488 CCB73011.1     32
 SCATT_02840   Streptomyces cattleya NRRL 8057 = DSM 46488 AEW92655.1     32
 BB341_20925   Streptomyces clavuligerus F613-1 ANW20495.1     32
 SCO7020 (AmlB)   Streptomyces coelicolor A3(2) CAB88153.1
NP_631084.1
Q9L035   32
 B446_11680   Streptomyces collinus Tu 365 AGS69158.1     32
 TU94_09625   Streptomyces cyaneogriseus subsp. noncyanogenus NMWT 1 AJP01730.1     32
 BN159_6199   Streptomyces davawensis JCM 4913 CCK30578.1     32
 BN159_6200 (AmY)   Streptomyces davawensis JCM 4913 CCK30579.1     32
 KY5_2222   Streptomyces formicae KY5 ATL27240.1     32
 SFUL_1791   Streptomyces fulvissimus DSM 40593 AGK76759.1     32
 B1H19_35195   Streptomyces gilvosporeus F607 ARF58738.1     32
 SGLAU_00940 (GacE2)   Streptomyces glaucescens GLA.O CAL64868.1
AIR96216.1
B0B0V6   32
 SGLAU_10130 (AmY)   Streptomyces glaucescens GLA.O AIR98036.1     32
 SGLAU_25410   Streptomyces glaucescens GLA.O AIS01020.1     32
 WQO_08900   Streptomyces globisporus C-1027 ALU93465.1     32
 AVL59_38160   Streptomyces griseochromogenes ATCC 14511 ANP54646.1     32
 α-amylase (Amy) 3.2.1.1 Streptomyces griseus IMRU3570 CAA40798.1 P30270   32
 SGR_5280   Streptomyces griseus subsp. griseus NBRC 13350 BAG22109.1 B1VZI4   32
 α-amylase 3.2.1.1 Streptomyces hygroscopicus AAA26698.1 P08486   32
 SHJG_3717   Streptomyces hygroscopicus subsp. jinggangensis 5008 AEY88989.1     32
 SHJGH_3482   Streptomyces hygroscopicus subsp. jinggangensis TL01 AGF63147.1     32
 SHL15_2267   Streptomyces hygroscopicus subsp. limoneus KCTC 1717 ALO93425.1     32
 SHXM_03764   Streptomyces hygroscopicus XM201 AQW50301.1     32
 ABB07_27185   Streptomyces incarnatus NRRL 8089 AKJ13584.1     32
 SIRAN7128   Streptomyces iranensis CDR10953.1     32
 SLA_1809   Streptomyces laurentii ATCC 31255 BAU82747.1     32
 Sle_49310   Streptomyces leeuwenhoekii C34 (= DSM 42122 = NRRL B-24963) CQR64389.1     32
 α-amylase (Aml) 3.2.1.1 Streptomyces limosus ATCC 19778 AAA88554.1 P09794   32
 SLINC_2556   Streptomyces lincolnensis NRRL 2936 ANS64780.1     32
 α-amylase (AmyA)   Streptomyces lividans ATCC 19844 ABZ81609.1 B0ZCN0   32
 α-amylase (Aml;AmlA) 3.2.1.1 Streptomyces lividans TK21 CAA73926.1 O86876   32
 α-amylase (AmlB) 3.2.1.1 Streptomyces lividans TK24 CAB06622.1 P97179   32
 SLIV_04145 (AmL)   Streptomyces lividans TK24 AIJ11852.1     32
 amylase (fragment)   Streptomyces lonarensis ADZ45287.1     32
 amylase   Streptomyces lonarensis NCL 716 ADZ45287.2     32
 SL103_22760   Streptomyces lydicus 103 AOP48684.1     32
 SMALA_2143   Streptomyces malaysiensis DSM 4137 ATL82377.1     32
 BBN63_25315   Streptomyces niveus SCSIO 3406 AQU69006.1     32
 SNOD_30550   Streptomyces nodosus ATCC 14899 AJE43854.1     32
 SNOUR_08095   Streptomyces noursei ATCC 11455 ANZ14938.1     32
 B1H29_04330   Streptomyces pactum ACT12 AQS66264.1     32
 B1H29_26265   Streptomyces pactum ACT12 AQS69916.1     32
 BC342_08765   Streptomyces pactum KLBMP 5084 AOW86628.1     32
 Spa2297_09300   Streptomyces parvulus 2297 ANJ07185.1     32
 CGZ69_28340   Streptomyces peucetius subsp. caesius ATCC 27952 ATW51177.1     32
 LK06_008085   Streptomyces pluripotens MUSC 135 ARP69930.1     32
 LK07_09185   Streptomyces pluripotens MUSC 137 ASN24185.1     32
 Sfla_4612   Streptomyces pratensis ATCC 33331 ADW06013.1 C9NEV1   32
 SPRI_1863   Streptomyces pristinaespiralis HCCB 10218 ALC20169.1     32
 BFF78_30055   Streptomyces puniciscabiei TW1S1 AOR37415.1     32
 M271_35280   Streptomyces rapamycinicus NRRL 5491 AGP58463.1     32
 TUE45_06959 (Aml_3)   Streptomyces reticuli CUW32210.1     32
 TUE45_02766 (Aml_1)   Streptomyces reticuli CUW28036.1     32
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