Activities in Sub Family	[retaining] α-transglucosidase / α-glucosyltransferase (EC 2.4.1.-); Oligosaccharide α-4-glucosyltransferase (EC 2.4.1.161); Palatinase (EC 3.2.1.-); [retaining] α-amylase (EC 3.2.1.1); [retaining] oligo-α-1,6-glucosidase (EC 3.2.1.10); α-glucosidase (EC 3.2.1.20); Glucodextranase (EC 3.2.1.70); [retaining] isomaltulose synthase / sucrose isomerase / sucrose glucosylmutase (EC 5.4.99.11);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (2); Uniprot accession (39); PDB accession (49); 3D entries (13); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
oligo-α-1,6-glucosidase (MalL)	3.2.1.10	Bacillus cereus ATCC 7064	CAA37583.1	P21332	1UOK[A] 2.00	31
α-glucosidase with high transglucosylation activity (BspAG13_31A)	3.2.1.20	Bacillus sp. (in: firmicutes) AHU2216	BBB94188.1	A0A2Z5WH92	5ZCB[A] 2.50 5ZCC[A] α-D-Glcp-(1-4)-α-D-Glcp 1.70 5ZCD[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.71 5ZCE[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.56 8IBK[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.69 8IDS[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.50	31
α-1,6-glucosidase (MalL;YvdL;BSU34560)	3.2.1.10	Bacillus subtilis subsp. subtilis str. 168	CAB15461.1 AIY94772.1 AQR83490.1 AQR87705.1 CAB08041.1 NP_391336.1	A0A6M4JPF4 O06994	4M56[A,B] 2.30 4M8U[A] 1.45 4MAZ[A] 1.60 4MB1[A] 1.40 5WCZ[A,B] <carb_like_NOJ> 1.58 7LV6[A] 1.10	31
sucrose isomerase / trehalulose synthase (MutB)	5.4.99.11	Burkholderia ubonensis subsp. mesacidophila MX-45	ABC33903.1 AAY69574.1 ABT00107.1 ACO05018.1 CAF32995.1	M1E1F3 M1E1F6 M1E1F7 Q2PS28	1ZJA[A,B] 1.60 1ZJB[A,B] 1.80 2PWD[A,B] <carb_like_NOJ> 1.80 2PWE[A,B] α-D-Glcp-(1-2)-β-D-Fruf 2.00 2PWF[A,B,C,D] β-D-Glcp 1.80 2PWG[A,B] <carb_like_CTS> 2.20 2PWH[A,B] 2.00 4GI6[A,B] α-D-Glcp 2.15 4GI8[A,B] 1.95 4GI9[A,B] 2.15 4GIA[A,B] 2.01 4GIN[A] 1.90 4GO8[A,B] 2.15 4GO9[A,B] 2.20 4H2C[A] 1.70 4H7V[A,B] α-D-Glcp 1.80 4H8H[A,B] 2.00 4H8U[A,B] α-D-Glcp-(1-1)-<non_carb> α-D-Glcp-(1-2)-β-D-Fruf 2.00 4H8V[A,B] α-D-Glcp-(1-1)-<non_carb> 1.95 4HA1[A,B] α-D-Glcp α-D-Glcp-(1-1)-<non_carb> 2.20	31
isomaltulose synthase (PalI)	5.4.99.11	Erwinia rhapontici NX-5 / DSM 4484	AAK28735.1 AAY69569.1 ACW35803.1 ADJ56407.2 CAC69266.1 CAC69272.1 CAC69285.1 CAF32986.1 CAF32987.1	D9MPF2 Q9AI64	4HOW[A] 1.70 4HOX[A] 2.00 4HOZ[A] α-D-Glcp 2.00 4HP5[A] α-D-Glcp 2.00 4HPH[A] α-D-Glcp-(1-2)-β-D-Fruf 1.70	31
α-glucosidase (Gsj)	3.2.1.20	Geobacillus sp. HTA-462	BAE48285.1	Q33E90	2ZE0[A] 2.00	31
isomaltulose synthase (PalI)	5.4.99.11	Klebsiella sp. LX3	AAK82938.1 CAF32992.1 CAF32993.1 CBF79192.1	Q8KR84	1M53[A] 2.20	31
oligo-α-1,6-glucosidase (LaGH13_31A; LBA0264)	3.2.1.10	Lactobacillus acidophilus NCFM	AAV42157.1	Q5FMB7	4AIE[A] 2.05	31
α-1,4-glucosyltransferase (LaGH13_31B; MalL; LBA1872)	3.2.1.1 2.4.1.161	Lactobacillus acidophilus NCFM	AAV43672.1 ACC31273.1 ACS07503.1 ACS09974.1	Q5FI02	6Y9T[A,B] 2.78	31
α-1,6-glucosidase (Lmo0184)	3.2.1.20	Listeria monocytogenes EGD-e	NP_463715.1 CAC98399.1 CAZ78714.1	Q8YAE6	5DO8[A,B,C] β-D-Glcp 1.80	31
sucrose isomerase (SmuA)	5.4.99.11	Protaminobacter rubrum CBS 547.77	CAF32988.1 AAE36427.1 AAY69568.1 AAY69572.1 ACW35802.1 ACW35805.1 CAF32985.1	D0VX20	3GBD[A] 1.95 3GBE[A] <carb_like_NOJ> 1.70	31
dextran α-1,6-glucosidase (DexB;SmDG)	3.2.1.70	Streptococcus mutans ATCC 25175	BAE79634.1	Q2HWU5 Q99040	2ZIC[A] 2.20 2ZID[A] α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-α-D-Glcp 2.20	31
dextran α-1,6-glucosidase (DexB;SMU.883)		Streptococcus mutans UA159	AAN58598.1 NP_721292.1		4WLC[A] β-D-Glcp-(1-4)-ASP 2.40 4XB3[A] 2.09	31
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