Activities in Sub Family	[retaining] α-amylase (EC 3.2.1.1);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (27); Uniprot accession (27); PDB accession (3); 3D entries (2); cryst (0)

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Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
I9X38_06345		Bacillus mojavensis PS17	QQF63899.1			28
N2384_05980		Bacillus paralicheniformis NG-ABK-31	UWS62582.1			28
M0696_01765 (AmyE)		Bacillus rugosus A78.1	UPV79512.1			28
JD965_01600		Bacillus siamensis B28	QQD82364.1			28
IXY25_10975		Bacillus siamensis BZR 86	QPG16591.1			28
LYZ33_01615		Bacillus siamensis GUWM35	UIO92856.1			28
KI431_01585		Bacillus siamensis IMD4001	UXZ18250.1			28
CWD84_20345		Bacillus siamensis SCSIO 05746	AUJ78981.1			28
NNG64_01615		Bacillus siamensis WYJ-E14	UUA84562.1			28
OM992_01635		Bacillus siamensis YB-1631	UZD74451.1			28
BS34A_03590 (AmyE)		Bacillus sp. (in: firmicutes) BS34A	CEJ75847.1			28
α-amylase		Bacillus sp. (in: firmicutes) ES05	QTZ83335.1			28
α-amylase, partial		Bacillus sp. (in: firmicutes) H3	WOT19242.1			28
amylase, partial (fragment)		Bacillus sp. (in: firmicutes) L-56	AWX67689.1			28
BZ167_08085		Bacillus sp. 275	AQP95972.1			28
GNE05_01950		Bacillus sp. AM1(2019)	QHJ02085.1			28
α-amylase		Bacillus sp. B-5	ADB81848.1			28
P3L57_01780		Bacillus sp. B28	WEY45482.1			28
α-amylase (AmyE)		Bacillus sp. BBM1	ADF47479.1			28
LK685_19960 (AmyE)		Bacillus sp. BC1-43	UEG57051.1			28
OY17_04305		Bacillus sp. BH072	AJE77362.1			28
DS740_01750		Bacillus sp. DM2	AXF31649.1			28
α-amylase		Bacillus sp. DR90	AGX26265.1			28
K3G25_15280		Bacillus sp. FCW2	UHC64107.1			28
BGM23_20350		Bacillus sp. FJAT-14266	AOL28808.1			28
α-amylase		Bacillus sp. FW1	BBA72323.1			28
amylase		Bacillus sp. HYC-1-3	ADH93705.1			28
JNUCC22_01610		Bacillus sp. JNUCC-22	QWQ28733.1			28
MY9_0311		Bacillus sp. JS	AFI26850.1			28
FFE90_001810 (AmyE)		Bacillus sp. KBS0812	QDW04005.1			28
α-amylase (Kra2;PKA)	3.2.1.1	Bacillus sp. KR8104	ACD93218.3 ACD93218.2	B3GQD0	3DC0[A]	28
JQX68_01770		Bacillus sp. LJBS06	QRZ93264.1			28
KXZ66_01705 (AmyE)		Bacillus sp. LJBS17	QXW82098.1			28
J6600_01590		Bacillus sp. LJBV19	QTN96029.1			28
BsLM_0316		Bacillus sp. LM 4-2	AKE22115.1			28
CS547_01600		Bacillus sp. Lzh-5	ATV21519.1			28
MAK48_01775 (AmyE)		Bacillus sp. Man122	UKS39138.1			28
C1T29_02055		Bacillus sp. MBGLi79	AUZ37160.1			28
CDO84_00780		Bacillus sp. MD-5	ASB59623.1			28
VP472_01625		Bacillus sp. MT(2024)	WVN54952.1			28
H7F27_19205		Bacillus sp. PAMC26543	QNH39607.1			28
SB24_08165		Bacillus sp. Pc3	AJC25153.1			28
L3V45_01600		Bacillus sp. R45	UJX18647.1			28
AUL54_09165		Bacillus sp. SDLI1	AME06508.1			28
RUI02_01745 (AmyE)		Bacillus sp. TSA-4	WNX00489.1			28
D9C22_01755		Bacillus sp. WR11	QAT73362.1			28
QF06_00475		Bacillus sp. YP1	AJO57006.1			28
EO946_01800		Bacillus spizizenii ATCC 6633 = JCM 2499	QCY15891.1			28
QR321_01790 (AmyE)		Bacillus spizizenii E25-3	WJD92859.1			28
V6D45_01745 (AmyE)		Bacillus spizizenii kcgeb_S11	WWO10193.1			28
SD85_01710		Bacillus spizizenii NRS 231	AJD35026.1			28
BSUW23_01555 (AmyE)		Bacillus spizizenii str. W23	ADM36368.1			28
DQ231_01660		Bacillus spizizenii SW83	AXC51632.1			28
GYO_0508		Bacillus spizizenii TU-B-10	AEP85220.1			28
QI003_01755 (AmyE)		Bacillus stercoris BS21	WGV95823.1			28
BSB_21600 (AmyE)		Bacillus stercoris BST19	BEV39087.1			28
QA442_20830 (AmyE)		Bacillus stercoris Mal05	WGE38783.1			28
QPZ67_11980		Bacillus stercoris SMPL712	WIL34284.1			28
α-amylase		Bacillus subtilis	AAQ83841.1 CAJ01439.1			28
α-amylase		Bacillus subtilis 233	AAZ30064.1			28
α-amylase	3.2.1.1	Bacillus subtilis 2633	CAA30643.1	Q45520	1BAG[A] 1UA7[A]	28
Bateq7PJ16_0365		Bacillus subtilis 7PJ-16	QHF56171.1			28
α-amylase (Amy28)	3.2.1.1	Bacillus subtilis A28	AFQ31615.1	J9QPI9		28
α-amylase (fragment)		Bacillus subtilis AL-2	AGN29207.1			28
F0366_20300 (AmyE)		Bacillus subtilis AMR1	QIR20414.1			28
α-amylase		Bacillus subtilis AS01a	AGC23389.1			28
HC662_03460 (AmyE)		Bacillus subtilis At3	QJC87232.1			28
BS11774_07665		Bacillus subtilis ATCC 11774	QAR60391.1			28
KS07_01540		Bacillus subtilis ATCC 19217	AIW36239.1			28
MA22_16150		Bacillus subtilis B-1	AIU77970.1			28
α-amylase, partial (fragment)		Bacillus subtilis b.s.y.12	AIU39224.1			28
BEST7003_0303 (AmyE)		Bacillus subtilis BEST7003	BAM56504.1			28
α-amylase (AmyE)	3.2.1.1	Bacillus subtilis BF7658	ACK37366.1	B8Y1H0		28
C0W65_11610		Bacillus subtilis BJ3-2	AUS12602.1			28
GTW28_01760 (AmyE)		Bacillus subtilis BJQ0005	QHH18731.1			28
KO64_01645		Bacillus subtilis Bs-916	AIW28644.1			28
BS16045_00352 (AmyA)		Bacillus subtilis BS16045	AOL96105.1			28
BSBS38_00352 (AmyA)		Bacillus subtilis BS38	AOR96667.1			28
BS49_03590 (AmyE)		Bacillus subtilis BS49	CEI55422.1			28
BSn5_13100		Bacillus subtilis BSn5	ADV95234.1			28
α-amylase (Amy7C;Amy7D) (fragment)	3.2.1.1	Bacillus subtilis CN7	AFC90847.1	H9B4I9		28
α-amylase (Amy7)		Bacillus subtilis CN7	AFD33644.1			28
BCV50_11095		Bacillus subtilis CW14	ARV45526.1			28
alpha amylase, partial (fragment)		Bacillus subtilis D5-2	AND80846.1			28
α-amylase		Bacillus subtilis DR8806	AGW23675.1			28
DTQ03_01735		Bacillus subtilis FB6-3	AXV60171.1			28
FIU26_16795		Bacillus subtilis FDAARGOS_606	QDD05375.1			28
α-amylase (AmyA)		Bacillus subtilis FP-133	BAT21551.1			28
CD007_01755		Bacillus subtilis ge25	ASB98155.1			28
GSY53_09585 (AmyE)		Bacillus subtilis GOT9	QHE16056.1			28
BSK2_01750		Bacillus subtilis GQJK2	ARB35747.1			28
α-amylase (Amy) (fragment)		Bacillus subtilis H1	AIY53013.1			28
α-amylase	3.2.1.1	Bacillus subtilis HA401	AAT01440.1	Q6PMJ3		28
BSHJ0_00347 (AmyA)		Bacillus subtilis HJ0-6	AOA52954.1			28
AW03_002990		Bacillus subtilis HJ5	AKD33705.1			28
amylase		Bacillus subtilis HLSSK-3	ADH93704.1			28
D0819_18705		Bacillus subtilis HMNig-2	QFY87265.1			28
amylase		Bacillus subtilis I527	ADH93706.1			28
BHE96_01635		Bacillus subtilis J-5	APH34368.1			28
HR084_01815 (AmyE)		Bacillus subtilis JCL16	QKJ76429.1			28
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