Activities in Sub Family	[retaining] cyclic α-1,6-maltosyl-maltose hydrolase (EC 3.2.1.-); [retaining] α-amylase (EC 3.2.1.1); [retaining] maltogenic α-amylase (EC 3.2.1.133); [retaining] neopullulanase (EC 3.2.1.135); [retaining] pullulanase (EC 3.2.1.41); [retaining] cyclomaltodextrinase (EC 3.2.1.54);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (7); Uniprot accession (59); PDB accession (42); 3D entries (12); cryst (0)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
α-amylase / cyclomaltodextrinase (PFTA; PF1939) (Amy13)	3.2.1.1 3.2.1.54	Pyrococcus furiosus DSM 3638	AAL82063.1 CAZ78721.1 NP_579668.1	Q8TZP8	4AEF[A,B] 2.34	20
maltogenic α-amylase / cyclomaltodextrinase (MA;SmMA;Smar_0613)	3.2.1.133 3.2.1.54	Staphylothermus marinus F1	ABN69720.1	A3DM60	4AEE[A,B] 2.28	20
amylopullulanase / pullulanase type III (Pul-Tk;TK-PUL;TK0977)	3.2.1.1 3.2.1.41	Thermococcus kodakarensis KOD1	BAD85166.1 CAT69621.1 CAZ78793.1	Q5JID9	5OT1[A] 2.80	20
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
α-amylase (Amy703;Amy)	3.2.1.1	Alkalihalophilus pseudofirmus 703	AGT54942.1	A0A023I4U3	8JNX[A] 3.20	20
cyclic α-1,6-maltosyl-maltose hydrolase (CmmF)	3.2.1.-	Arthrobacter globiformis M6	BAI67607.1	D2YYE1	5ZXG[A,B] 2.40 6A0J[A] 1.60 6A0K[A,B] 1.94 6A0L[A] 2.10	20
cyclomaltodextrinase (CDI5;BspCMD)	3.2.1.54	Bacillus sp. (in: firmicutes)	AAA92925.1	Q59226	1EA9[C] 3.2	20
cyclomaltodextrinase / neopullulanase (NplT;BsCMD)	3.2.1.135 3.2.1.54	Geobacillus stearothermophilus TRS40	AAA22622.1	P38940	1J0H[A,B] 1.90 1J0I[A,B] α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp 2.40 1J0J[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.80 1J0K[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-6)-α-D-Glcp 3.20	20
debranching enzyme (Npun_R6073)	3.2.1.41	Nostoc punctiforme PCC 73102	ACC84362.1 CAY02945.1 CAZ78799.1	B2IUW9	2WC7[A] 2.37 2WCS[A] 2.80 2WKG[A] 3.00	20
cyclomaltodextrinase / neopullulanase (TVA II;TvCMD)	3.2.1.135 3.2.1.54	Thermoactinomyces vulgaris R-47	BAA02473.1 BAA97040.1 BAB40638.1 CAY02972.1 CAZ78903.1	Q08751 Q7DI93	1BVZ[A,B] 2.60 1G1Y[A,B] b-cyclodextrin 3.00 1JF5[A,B] 3.20 1JF6[A,B] 3.20 1JI2[A,B] 2.30 1JIB[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 3.30 1JL8[A,B] b-cyclodextrin 3.20 1VB9[A,B] α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)[α-D-Glcp-(1-6)]-α-D-Glcp-(1-4)-α-D-Glcp 2.20 1VFK[A,B] α-D-Glcp α-D-Glcp-(1-4)-D-1-deoxy-Glcp β-D-Glcp-(1-4)-α-D-Glcp 2.90 1VFM[A,B] 2.90 1VFO[A,B] 2.81 1VFU[A,B] 3.10 1WZK[A,B] 2.30 1WZL[A,B] 2.00 1WZM[A,B] 3.20 2D2O[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp 2.10 3A6O[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-<non_carb>-(0-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp 2.80	20
cyclomaltodextrinase / maltogenic α-amylase (ThCMD;ThMA)	3.2.1.133 3.2.1.54	Thermus sp. IM6501	AAC15072.1	O69007	1GVI[A] b-cyclodextrin 3.3 1SMA[A] 2.80	20
TTHA1563		Thermus thermophilus HB8	BAD71386.1		2Z1K[A,B,C,D] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp-(1-4)-ASP 2.30	20
reducing-end specific α-glucosidase (WcAG)	3.2.1.1	Weissella cibaria BBK-1	UGA57824.1	A0A482PRE5	7D9B[A] 1.58 7D9C[A] α-D-Glcp-(1-4)-α-D-Glcp 1.36 7DCG[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.53 7DCH[A] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.69 7EHH[A] α-D-Glcp-(1-4)-α-D-Glcp 2.00 7EHI[A] 1.69	20
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