Glycoside Hydrolase Family 13 / Subf 20

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (1649); Uniprot accession (285); PDB accession (30); 3D entries (8); cryst (0)
Archaea
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 α-amylase / cyclomaltodextrinase (PFTA;PF1939) (Amy13) 3.2.1.1
3.2.1.54
Pyrococcus furiosus DSM 3638 AAL82063.1
CAZ78721.1
NP_579668.1
Q8TZP8
4AEF[A,B] 2.34
20
 maltogenic α-amylase / cyclomaltodextrinase (MA;SmMA;Smar_0613) 3.2.1.133
3.2.1.54
Staphylothermus marinus F1 ABN69720.1 A3DM60
4AEE[A,B] 2.28
20
Bacteria
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 cyclomaltodextrinase (CDI5;BspCMD) 3.2.1.54 Bacillus sp. AAA92925.1 Q59226
1EA9[C] 3.2
20
 cyclomaltodextrinase / neopullulanase (NplT;BsCMD) 3.2.1.135
3.2.1.54
Geobacillus stearothermophilus TRS40 AAA22622.1 P38940
1J0H[A,B] 1.90
1J0I[A,B] α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp
2.40
1J0J[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.80
1J0K[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-6)-α-D-Glcp
3.20
20
 debranching enzyme (Npun_R6073) 2.4.1.25
3.2.1.33
Nostoc punctiforme PCC 73102 ACC84362.1
CAY02945.1
CAZ78799.1
B2IUW9
2WC7[A] 2.37
2WCS[A] 2.80
2WKG[A] 3.00
20
 cyclomaltodextrinase / neopullulanase (TVA II;TvCMD) 3.2.1.135
3.2.1.54
Thermoactinomyces vulgaris R-47 BAA02473.1
BAA97040.1
BAB40638.1
CAY02972.1
CAZ78903.1
Q08751
Q7DI93
1BVZ[A,B] 2.60
1G1Y[A,B] b-cyclodextrin
3.00
1JF5[A,B] 3.20
1JF6[A,B] 3.20
1JI2[A,B] 2.30
1JIB[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
3.30
1JL8[A,B] b-cyclodextrin
3.20
1VB9[A,B] α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)[α-D-Glcp-(1-6)]-α-D-Glcp-(1-4)-α-D-Glcp
2.20
1VFK[A,B] α-D-Glcp
α-D-Glcp-(1-4)-D-1-deoxy-Glcp
β-D-Glcp-(1-4)-α-D-Glcp
2.90
1VFM[A,B] 2.90
1VFO[A,B] 2.81
1VFU[A,B] 3.10
1WZK[A,B] 2.30
1WZL[A,B] 2.00
1WZM[A,B] 3.20
2D2O[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp
2.10
3A6O[A,B] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-<non_carb>-(0-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp
2.80
20
 cyclomaltodextrinase / maltogenic α-amylase (ThCMD;ThMA) 3.2.1.133
3.2.1.54
Thermus sp. IM6501 AAC15072.1 O69007
1GVI[A] b-cyclodextrin
3.3
1SMA[A] 2.80
20
 TTHA1563   Thermus thermophilus HB8 BAD71386.1 Q5SI17
2Z1K[A,B,C,D] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp-(1-4)-ASP
2.30
20
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