Glycoside Hydrolase Family 13 / Subf 20

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (1676); Uniprot accession (285); PDB accession (30); 3D entries (8); cryst (0)
Archaea
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 pullulanase (ApuA;Pul-Dm;Desmu_0653) 3.2.1.41 Desulfurococcus mucosus DSM 2162 AAG31033.1
ADV64961.1
E8R8Y5
Q9HHB0
  20
 α-amylase / cyclomaltodextrinase (PFTA;PF1939) (Amy13) 3.2.1.1
3.2.1.54
Pyrococcus furiosus DSM 3638 AAL82063.1
CAZ78721.1
NP_579668.1
Q8TZP8 4AEF[A,B] 20
 maltogenic α-amylase / cyclomaltodextrinase (MA;SmMA;Smar_0613) 3.2.1.133
3.2.1.54
Staphylothermus marinus F1 ABN69720.1 A3DM60 4AEE[A,B] 20
 pullulanase type III (PulhA;Pul-Ta) 3.2.1.41 Thermococcus aggregans CAB94218.1
CAZ78888.1
Q9P9A0   20
 cyclomaltodextrinase (CD;TcCD;CL1_0884) 3.2.1.54 Thermococcus cleftensis AFL95089.1     20
 cyclodextrinase (CDase-Tk;TK1770) 3.2.1.54 Thermococcus kodakarensis KOD1 BAD85959.1
CAT70564.1
Q5JJ59   20
 amylopullulanase / pullulanase type III (Pul-Tk;TK-PUL;TK0977) 3.2.1.1
3.2.1.41
Thermococcus kodakarensis KOD1 BAD85166.1
CAT69621.1
CAZ78793.1
Q5JID9   20
 cyclomaltodextrinase (Tocd;TON_1796) 3.2.1.54 Thermococcus onnurineus NA1 ACJ17286.1 B6YV58   20
 cyclomaltodextrinase (CdnA) 3.2.1.133
3.2.1.54
Thermococcus sp. B1001 BAB18100.1
CAZ78887.1
Q9HHC8   20
 maltogenic α-amylase / cyclomaltodextrinase (TfMA;Tpen_1458) 3.2.1.133
3.2.1.54
Thermofilum pendens Hrk 5 ABL78855.1 A1S075   20
 maltogenic α-amylase (TpMA;TVG0226058) 3.2.1.133 Thermoplasma volcanium GSS1 BAB59359.1
CAZ78693.1
NP_110736.1
Q97C86   20
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 cyclomaltodextrinase (CdaA;Aaci_2869) 3.2.1.54 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 ACV59873.1
CAB40078.1
CAB65655.1
CAY02967.1
CAZ78890.1
C8WUQ7
Q9WX32
  20
 cyclomaltodextrinase (Cda13;Amy98) 3.2.1.54 Anoxybacillus flavithermus AAX29991.1
CAY02963.1
CAZ78871.1
Q5BLZ6   20
 cyclic maltosyl-maltose hydrolase (CmmF) 3.2.1.- Arthrobacter globiformis M6 BAI67607.1 D2YYE1   20
 maltogenic α-amylase 3.2.1.133 Bacillus acidopullulyticus CAA80246.1
CAY02968.1
CAZ78895.1
P32818   20
 pullulanase (BFLpu) 3.2.1.41 Bacillus flavocaldarius KP1228 BAB18516.1 Q9F236   20
 maltogenic α-amylase (Mag1;BleG1_0772) 3.2.1.54 Bacillus lehensis G1 AHW51935.1
AIC93380.1
    20
 maltogenic α-amylase (BLMA) (frameshift) 3.2.1.133 Bacillus licheniformis CAA47612.1 Q04977   20
 α-amylase (Amy703;Amy) 3.2.1.1 Bacillus pseudofirmus 703 AGT54942.1     20
 cyclomaltodextrinase (CDI5;BspCMD) 3.2.1.54 Bacillus sp. AAA92925.1 Q59226 1EA9[C] 20
 cyclodextrinase 3.2.1.54 Bacillus sp. A2-5A BAA31576.1 O82982   20
 α-amylase (AmyL) 3.2.1.1 Bacillus sp. AAH-31 BAN63122.1     20
 neopullulanase 3.2.1.135 Bacillus sp. KSM-1876 BAA02521.1 Q57482   20
 maltogenic amylase (MAUS149) 3.2.1.133 Bacillus sp. US149 CAL64396.1 A7DWA8   20
 maltogenic α-amylase 3.2.1.133 Bacillus sp. WPD616 AAX85453.1 Q52PU5   20
 oligosaccharide-producing multifunctional amylase (OPMA-N) 3.2.1.135 Bacillus sp. ZW2531-1 ABY71030.1 B0FZ47   20
 maltogenic α-amylase / neopullulanase (YvdF;BSU34620) 3.2.1.133 Bacillus subtilis subsp. subtilis str. 168 CAB08035.1
AIY94778.1
AQR83496.1
AQR87711.1
CAB15467.1
NP_391342.1
O06988   20
 maltogenic amylase (BbmA) 3.2.1.133 Bacillus subtilis SUH4-2 AAF23874.1 Q9R9H8   20
 maltogenic amylase (BTMA) 3.2.1.133
3.2.1.54
Bacillus thermoalkalophilus ET2 AAT94159.1 Q68KL3   20
 α-amylase 2 (AmyB) 3.2.1.1 Dictyoglomus thermophilum CAA31586.1
CAZ78892.1
P14898   20
 maltogenic α-amylase (BSMA) 3.2.1.133 Geobacillus stearothermophilus ET1 AAC46346.1 Q45490   20
 neopullulanase 3.2.1.135 Geobacillus stearothermophilus IMA6503 AAK15003.1 Q9AIV2   20
 cyclomaltodextrinase / neopullulanase (NplT;BsCMD) 3.2.1.135
3.2.1.54
Geobacillus stearothermophilus TRS40 AAA22622.1 P38940 1J0H[A,B]
1J0I[A,B]
1J0J[A,B]
1J0K[A,B]
20
 maltogenic-amylase (Amy3;Gt-Mamy) 3.2.1.133 Geobacillus thermoleovorans NP54 AFM43699.1     20
 cyclodextrinase (CymH) 3.2.1.54 Klebsiella oxytoca M5A1 CAA60007.1
CAY02962.1
CAZ78869.1
Q48398   20
 cyclomaltodextrinase (Cda13;Amy80) 3.2.1.54 Laceyella sacchari AAX29990.1
CAY02964.1
CAZ78872.1
Q5BLZ7   20
 maltogenic α-amylase (LgMA;Lgas02001568) 3.2.1.133 Lactobacillus gasseri ATCC 33323 = JCM 1131 ZP_00047084.1 Q046J8   20
 maltogenic α-amylase (Lgma;LGAS_0215) 3.2.1.133 Lactobacillus gasseri ATCC 33323 = JCM 1131 ABJ59624.1 Q046J8   20
 cyclodextrinase (CDase) 3.2.1.54 Lysinibacillus sphaericus E-244 CAA44454.1
CAY02969.1
CAZ78898.1
Q08341   20
 debranching enzyme (Npun_R6073) 2.4.1.25
3.2.1.33
Nostoc punctiforme PCC 73102 ACC84362.1
CAY02945.1
CAZ78799.1
B2IUW9 2WC7[A]
2WCS[A]
2WKG[A]
20
 cyclodextrinase 3.2.1.54 Paenibacillus sp. A11 AAO47386.1
CAY02965.1
CAZ78882.1
Q84AP9   20
 maltogenic amylase (MA;GcaTK4MA) 3.2.1.133 Parageobacillus caldoxylosilyticus TK4 ACN79585.1 C0LZ63   20
 cyclomaltodextrinase / neopullulanase (TVA II;TvCMD) 3.2.1.135
3.2.1.54
Thermoactinomyces vulgaris R-47 BAA02473.1
BAA97040.1
BAB40638.1
CAY02972.1
CAZ78903.1
Q08751
Q7DI93
1BVZ[A,B]
1G1Y[A,B]
1JF5[A,B]
1JF6[A,B]
1JI2[A,B]
1JIB[A,B]
1JL8[A,B]
1VB9[A,B]
1VFK[A,B]
1VFM[A,B]
1VFO[A,B]
1VFU[A,B]
1WZK[A,B]
1WZL[A,B]
1WZM[A,B]
2D2O[A,B]
3A6O[A,B]
20
 cyclodextrinase (CDase) 3.2.1.54 Thermoanaerobacter ethanolicus ATCC 33223 / 39E AAA23219.1
CAY02970.1
CAZ78899.1
P29964   20
 cyclomaltodextrinase (AglB;TM1835) 3.2.1.54 Thermotoga maritima MSB8 AAD36898.1
AGL50768.1
AHD18274.1
AKE29585.1
CAY02913.1
CAZ78694.1
NP_229632.1
G4FGM1
Q9X2F4
  20
 cyclomaltodextrinase glucanotransferase (MalA;AglB) 3.2.1.54 Thermotoga neapolitana CAA08867.1
CAY02961.1
CAZ78866.1
O86959   20
 cyclomaltodextrinase / maltogenic α-amylase (ThCMD;ThMA) 3.2.1.133
3.2.1.54
Thermus sp. IM6501 AAC15072.1 O69007 1GVI[A]
1SMA[A]
20
 pullulanase (TTHpu;TTC1198) (fragment) 3.2.1.41 Thermus thermophilus HB27 AAS81540.1
CAZ78778.1
YP_005167.1
Q72ID4   20
 cyclodextrinase (Amy1) 3.2.1.54 uncultured bacterium ACA48223.1 B1PHW8   20
 neopullulanase (Amy29) 3.2.1.135 uncultured bacterium ACA48224.1 B1PHW9   20
 cyclodextrinase (Amy132) 3.2.1.54 uncultured bacterium ACA48225.1 B1PHX0   20
 cyclomaltodextrinase (CdaA) (Cda13A) 3.2.1.54 uncultured soil bacterium ABA39290.1 Q3HW59   20
unclassified
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 Npu193A 3.2.1.- uncultured microorganism ABS59068.1 A7LI67   20
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