Glycoside Hydrolase Family 13 / Subf 20

Activities in Familyα-amylase (EC; pullulanase (EC; cyclomaltodextrin glucanotransferase (EC; cyclomaltodextrinase (EC; trehalose-6-phosphate hydrolase (EC; oligo-α-glucosidase (EC; maltogenic amylase (EC; neopullulanase (EC; α-glucosidase (EC; maltotetraose-forming α-amylase (EC; isoamylase (EC; glucodextranase (EC; maltohexaose-forming α-amylase (EC; maltotriose-forming α-amylase (EC; branching enzyme (EC; trehalose synthase (EC; 4-α-glucanotransferase (EC; maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC ; sucrose phosphorylase (EC; malto-oligosyltrehalose trehalohydrolase (EC; isomaltulose synthase (EC; malto-oligosyltrehalose synthase (EC; amylo-α-1,6-glucosidase (EC; α-1,4-glucan: phosphate α-maltosyltransferase (EC; 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (1649); Uniprot accession (285); PDB accession (30); 3D entries (8); cryst (0)
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 ASAC_1074   Acidilobus saccharovorans 345-15 ADL19479.1 D9Q2E1   20
 CPM_1614   Cuniculiplasma divulgatum PM4 (=JCM 30641; =VKM B-2940) SJK85401.1     20
 CSP5_1645   Cuniculiplasma divulgatum S5(T) (=JCM 30642; =VKM B-2941) SIM79844.1     20
 DKAM_0273   Desulfurococcus amylolyticus 1221n ACL10599.1 B8D2L1   20
 Desfe_0644   Desulfurococcus amylolyticus DSM 16532 AFL66545.1     20
 Desmu_0336   Desulfurococcus mucosus DSM 2162 ADV64655.1     20
 pullulanase (ApuA;Pul-Dm;Desmu_0653) Desulfurococcus mucosus DSM 2162 AAG31033.1
 Hmuk_0209   Halomicrobium mukohataei DSM 12286 ACV46346.1 C7NWY1   20
 Halxa_4124   Halopiger xanaduensis SH-6 AEH38729.1     20
 PAP_04995   Palaeococcus pacificus DY20341 AIF69410.1     20
 PFC_08890   Pyrococcus furiosus COM1 AFN04701.1     20
 α-amylase / cyclomaltodextrinase (PFTA;PF1939) (Amy13)
Pyrococcus furiosus DSM 3638 AAL82063.1
Q8TZP8 4AEF[A,B] 20
 TQ32_06200   Pyrococcus kukulkanii NCB100 AMM54113.1     20
 PYCH_17070   Pyrococcus yayanosii CH1 AEH25366.1     20
 Shell_0233   Staphylothermus hellenicus DSM 12710 ADI31372.1 D7DB25   20
 maltogenic α-amylase / cyclomaltodextrinase (MA;SmMA;Smar_0613)
Staphylothermus marinus F1 ABN69720.1 A3DM60 4AEE[A,B] 20
 pullulanase type III (PulhA;Pul-Ta) Thermococcus aggregans CAB94218.1
Q9P9A0   20
 TBCH5v1_1915   Thermococcus barophilus CH5 ALM75820.1     20
 TERMP_01653   Thermococcus barophilus MP ADT84628.1     20
 A3L01_10085   Thermococcus barossii SHCK-94 ASJ05693.1     20
 A3L02_08135   Thermococcus celer Vu 13 ASI99531.1     20
 A3L02_04155   Thermococcus celer Vu 13 ASI99919.1     20
 cyclomaltodextrinase (CD;TcCD;CL1_0884) Thermococcus cleftensis AFL95089.1     20
 CL1_0091   Thermococcus cleftensis AFL94306.1     20
 TEU_03500   Thermococcus eurythermalis A501 AIU69487.1     20
 TGAM_0599   Thermococcus gammatolerans EJ3 ACS33101.1 C5A4D9   20
 TGAM_1752   Thermococcus gammatolerans EJ3 ACS34254.1 C5A1I5   20
 A3K92_06290   Thermococcus gorgonarius W-12 ASJ01118.1     20
 A3K92_02180   Thermococcus gorgonarius W-12 ASJ00372.1     20
 X802_01660   Thermococcus guaymasensis DSM 11113 AJC71037.1     20
 X802_07225   Thermococcus guaymasensis DSM 11113 AJC71972.1     20
 cyclodextrinase (CDase-Tk;TK1770) Thermococcus kodakarensis KOD1 BAD85959.1
Q5JJ59   20
 amylopullulanase / pullulanase type III (Pul-Tk;TK-PUL;TK0977)
Thermococcus kodakarensis KOD1 BAD85166.1
Q5JID9   20
 OCC_09349   Thermococcus litoralis DSM 5473 EHR79590.1     20
 cyclomaltodextrinase (Tocd;TON_1796) Thermococcus onnurineus NA1 ACJ17286.1 B6YV58   20
 A3L08_07175   Thermococcus pacificus P-4 ASJ07670.1     20
 A3L08_02825   Thermococcus pacificus P-4 ASJ06341.1     20
 TES1_1658   Thermococcus paralvinellae ES1 AHF81034.1     20
 A0127_00260   Thermococcus peptonophilus OG-1 AMQ17709.1     20
 A3L09_07315   Thermococcus profundus DT 5432 ASJ03075.1     20
 A3L10_09460   Thermococcus radiotolerans EJ2 ASJ15344.1     20
 A3L11_07945   Thermococcus siculi RG-20 ASJ09163.1     20
 A3L11_03375   Thermococcus siculi RG-20 ASJ08321.1     20
 ADU37_CDS18980   Thermococcus sp. 2319x1 ALV63597.1     20
 ADU37_CDS20530   Thermococcus sp. 2319x1 ALV63752.1     20
 GQS_06205 (fragment)   Thermococcus sp. 4557 AEK73139.1     20
 CDI07_03875   Thermococcus sp. 5-4 ASA77467.1     20
 CDI07_09920   Thermococcus sp. 5-4 ASA78605.1     20
 TAM4_475   Thermococcus sp. AM4 EEB74530.1     20
 TAM4_1607   Thermococcus sp. AM4 EEB74240.2     20
 cyclomaltodextrinase (CdnA)
Thermococcus sp. B1001 BAB18100.1
Q9HHC8   20
 A3L12_05090   Thermococcus sp. P6 ASJ10715.1     20
 A3L14_00955   Thermococcus thioreducens OGL-20P ASJ11544.1     20
 A3L14_05815   Thermococcus thioreducens OGL-20P ASJ12437.1     20
 N186_08320   Thermofilum adornatus 1910b AGT36002.1     20
 maltogenic α-amylase / cyclomaltodextrinase (TfMA;Tpen_1458)
Thermofilum pendens Hrk 5 ABL78855.1 A1S075   20
 MA03_06280   Thermofilum uzonense 1807-2 AKG38933.1     20
 TCELL_0558   Thermogladius calderae 1633 AFK50983.1     20
 TCELL_1285   Thermogladius calderae 1633 AFK51708.1     20
 Ta0138   Thermoplasma acidophilum DSM 1728 CAC11285.1
Q9HLT7   20
 maltogenic α-amylase (TpMA;TVG0226058) Thermoplasma volcanium GSS1 BAB59359.1
Q97C86   20
 Tagg_0445   Thermosphaera aggregans DSM 11486 ADG90720.1 D5U0S0   20
 Tagg_0897   Thermosphaera aggregans DSM 11486 ADG91169.1 D5U219   20

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