Glycoside Hydrolase Family 13 / Subf 1

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (280); Uniprot accession (86); PDB accession (9); 3D entries (5); cryst (0)
Eukaryota
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 α-amylase (partial) 3.2.1.1 Aspergillus niger   P56271
2AAA[A] 2.10
1
 α-amylase A / B (AmyA;AmyB;An05g02100) 3.2.1.1 Aspergillus niger CBS 513.88 CAK44871.1
CAK46324.1
CAL33800.1
A2QL05
A2QL05
2GUY[A] 1.59
2GVY[A,B] α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-β-D-Glcp
1.80
1
 α-amylase (AmyB;TAA;Amy1;Amy2;Amy3;Asp o 21;Aspo21) 3.2.1.1 Aspergillus oryzae DSM63303 CAA31218.1
AAA32708.1
ACP34134.1
BAA00336.1
CAA31219.1
CAA31220.1
CAC40348.1
CAT03377.1
CAV35984.1
P0C1B3
P10529
2TAA[A] 3.00
3KWX[A] 2.40
6TAA[A] 2.10
7TAA[A] α-D-6-deoxy-Glcp-(1-4)-α-D-Glcp-(1-4)-<non_carb>-(0-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-β-D-6-deoxy-Glcp
1.98
1
 α-amylase (Amy1;AmyI;Taα-G1;AO090023000944;AmyA;AmyB) 3.2.1.1 Aspergillus oryzae RIB40 BAE59434.1 P0C1B3
3VX0[A] 1.50
1
 α-amylase (AmyA;McAmyA) 3.2.1.1 Malbranchea cinnamomea AFD54462.1  
3VM7[A] α-D-Glcp
2.25
1
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