Glycoside Hydrolase Family 13 / Subf 1

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (280); Uniprot accession (86); PDB accession (9); 3D entries (5); cryst (0)
| 1 | 2 |
Eukaryota
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 secreted protein   Achlya hypogyna ATCC 48635 AIG56379.1     1
 α-amylase (AmyAa) (fragment)   Aspergillus affinis ATCC MYA-4773 AFU52692.1     1
 α-amylase (Amyl III) 3.2.1.1 Aspergillus awamori KT-11 BAD06003.1
BAD06005.1
Q76L96   1
 α-amylase (Amyl I) 3.2.1.1 Aspergillus awamori KT-11 BAD06002.1 Q76L99   1
 alpha amylase   Aspergillus flavus APT42869.1     1
 alpha amylase   Aspergillus flavus APT42868.1     1
 α-amylase (Amy1;TAA) 3.2.1.1 Aspergillus flavus AAF14264.1
AAS89853.1
AAS89854.1
AAS89855.1
AAS89856.1
AAS89857.1
AAS89858.1
AAS89859.1
AAS89860.1
AAS89861.1
AAS89862.1
AAS89863.1
AAS89864.1
AAS89865.1
AAS89866.1
AAS89867.1
AAS89868.1
AAS89869.1
AAS89870.1
AAS89871.1
AAS89872.1
AAS89873.1
AAS89874.1
AAS89875.1
AAS89876.1
AAS89877.1
AAS89878.1
AAS89879.1
AAS89880.1
AAS89881.1
AAS89882.1
AAS89883.1
AAS89884.1
AAS89885.1
AAS89886.1
AAS89887.1
AAS89888.1
AAS89889.1
AAS89890.1
AAS89891.1
AAS89892.1
Q7LV45
Q96TH4
  1
 Afu4g10130   Aspergillus fumigatus Af293 XP_751813.1 Q4WPQ9   1
 α-amylase (AmyA) 3.2.1.1 Aspergillus kawachii IFO 4308 BAD01051.1 Q76CT3   1
 α-amylase (AsaA) 3.2.1.1 Aspergillus kawachii IFO 4308 BAA22993.1
CAX51628.1
O13296   1
 AN4507.2 (AmyC) (fragment)   Aspergillus nidulans FGSC A4 EAA60850.1 C8V8C6
Q5B4M3
  1
 AN3308.2 (AmyD)   Aspergillus nidulans FGSC A4 EAA63276.1 C8VHZ3
Q5B822
  1
 AN6324.2 (AmyE)   Aspergillus nidulans FGSC A4 EAA58708.1 C8V166
Q5AZF6
  1
 AN3388.2 (AmyF)   Aspergillus nidulans FGSC A4 EAA63356.1 C8VHN4
Q5B7U2
  1
 α-amylase (AmyA;AN2018.2) 3.2.1.1 Aspergillus nidulans FGSC A4 EAA64850.1
AAF17103.1
C8VLE6
G5EB45
  1
 α-amylase (AmyB;AN3402.2) 3.2.1.1 Aspergillus nidulans FGSC A4 AAF17100.1
EAA63370.1
C8VHL8
G5EAT0
Q9UV09
  1
 α-amylase, partial   Aspergillus niger AMX73966.1     1
 α-amylase (partial) 3.2.1.1 Aspergillus niger   P56271 2AAA[A] 1
 α-amylase A / B (AmyA;AmyB;An05g02100) 3.2.1.1 Aspergillus niger CBS 513.88 CAK44871.1
CAK46324.1
CAL33800.1
A2QL05
A2QL05
2GUY[A]
2GVY[A,B]
1
 acid α-amylase (AamA;An11g03340) 3.2.1.1 Aspergillus niger CBS 513.88 CAL15439.1
A35282
ADX42122.1
CAK48325.1
CAO78410.1
CAX51630.1
A2QW02   1
 An04g06930 (AmyC)   Aspergillus niger CBS 513.88 CAK44693.1 A2QJF8   1
 cell-wall 4-α-glucanotransferase (AgtA;An09g03100) 2.4.1.25 Aspergillus niger CBS 513.88 CAK40249.1 A2QTS4   1
 4-α-glucanotransferase (AgtB;An12g02460) 2.4.1.25 Aspergillus niger CBS 513.88 CAK41088.1 A2QYT9   1
 4-α-glucanotransferase (AgtC;An15g07800)   Aspergillus niger CBS 513.88 CAK42667.1 A2R6F9   1
 rutin α-L-rhamnosidase (RhaR)   Aspergillus niger DLFCC-90 ABW81206.1     1
 α-amylase A (AmyA) 3.2.1.1 Aspergillus niger VAR. AWAMORI UVK143F CAA36966.1 Q02905   1
 α-amylase B (AmyB) 3.2.1.1 Aspergillus niger VAR. AWAMORI UVK143F CAA36967.1
CAL15437.1
CAL15438.1
CAT03384.1
Q02906   1
 unnamed protein product (partial)   Aspergillus oryzae CAT03380.1     1
 unnamed protein product (partial)   Aspergillus oryzae CAT03381.1     1
 unnamed protein product   Aspergillus oryzae CAT03382.1     1
 unnamed protein product   Aspergillus oryzae CAT03383.1     1
 Sequence from patent 3.2.1.1 Aspergillus oryzae AAE13949.1
AAE37103.1
AAN27447.1
    1
 dioscin α-L-rhamnosidase (RhaD)   Aspergillus oryzae DLFCC-38 ADK23803.1 D9J2M5   1
 ginsenoside-α-L-rhamnosidase (RhaG)   Aspergillus oryzae DLFCC-39 ABY71841.1 B0FZ76
P0C1B4
  1
 α-amylase (AmyB;TAA;Amy1;Amy2;Amy3;Asp o 21;Aspo21) 3.2.1.1 Aspergillus oryzae DSM63303 CAA31218.1
AAA32708.1
ACP34134.1
BAA00336.1
CAA31219.1
CAA31220.1
CAC40348.1
CAT03377.1
CAV35984.1
P0C1B3
P10529
2TAA[A]
3KWX[A]
6TAA[A]
7TAA[A]
1
 AO090003001498   Aspergillus oryzae RIB40 BAE58540.1 Q2UIS5   1
 α-amylase (Amy1;AmyI;Taα-G1;AO090023000944;AmyA;AmyB) 3.2.1.1 Aspergillus oryzae RIB40 BAE59434.1 P0C1B3 3VX0[A] 1
 AO090120000263 (fragment)   Aspergillus oryzae RIB40 BAE62865.1 Q2U6F0   1
 α-amylase (Amy2;AmyII;Taα-G2;AO090120000196;AmyA;AmyB) 3.2.1.1 Aspergillus oryzae RIB40 BAE62808.1 P0C1B3   1
 α-amylase (Amy3;AmyIII;Taα-G3;AO090003001210;AmyA) 3.2.1.1 Aspergillus oryzae RIB40 BAA95703.1 P0C1B4   1
 α-amylase (Amy) 3.2.1.1 Aspergillus shirousami BAA01255.1 P30292   1
 α-amylase (TaaG1;AsTaaG1)   Aspergillus sojae KBN 1340 BAM28635.1     1
 α-amylase (AmyA) 3.2.1.1 Aspergillus tubingensis T8.4 AEB80431.1     1
 α-amylase   Aureobasidium pullulans NRRL Y-12974 AEH03024.1     1
 α-amylase   Aureobasidium pullulans P16 AOO85390.1     1
 α-amylase (AmyA)   Bispora sp. MEY-1 ADZ99364.1     1
 glycoside hydrolase family 13 protein (Bofut4_p008540.1)   Botrytis cinerea T4 CCD45137.1     1
 glycoside hydrolase family 13 protein (Bofut4_p114570.1)   Botrytis cinerea T4 CCD46751.1     1
 glycoside hydrolase family 13 protein (Bofut4_p035060.1)   Botrytis cinerea T4 CCD48213.1     1
 glycoside hydrolase family 13 protein (Bofut4_p108490.1)   Botrytis cinerea T4 CCD48465.1     1
 glycoside hydrolase family 13 protein (Bofut4_p018240.1)   Botrytis cinerea T4 CCD51518.1
CCD51518.2
    1
 CGB_B1340C   Cryptococcus gattii WM276 ADV20192.1     1
 CGB_E4540C   Cryptococcus gattii WM276 ADV22582.1     1
 CGB_J0550C   Cryptococcus gattii WM276 ADV24576.1     1
 CGB_K1010W   Cryptococcus gattii WM276 ADV25090.1     1
 CNAG_00122   Cryptococcus neoformans var. grubii H99 AFR92258.1     1
 CNAG_02189   Cryptococcus neoformans var. grubii H99 AFR95631.1     1
 CNAG_05264   Cryptococcus neoformans var. grubii H99 AFR94522.1
AGV14255.1
    1
 CNAG_01940   Cryptococcus neoformans var. grubii H99 AFR98135.1
AGV14739.1
    1
 CNL03660   Cryptococcus neoformans var. neoformans JEC21 AAW46412.1 Q5K924   1
 CNK02760   Cryptococcus neoformans var. neoformans JEC21 AAW46356.1 Q5K993   1
 CNH03660   Cryptococcus neoformans var. neoformans JEC21 AAW45221.1 Q5K924   1
 CNA01120   Cryptococcus neoformans var. neoformans JEC21 AAW41268.1 Q5KPY6   1
 CNE03480   Cryptococcus neoformans var. neoformans JEC21 AAW43607.1 Q5KGI6   1
 α-amylase (Amy-CS2) 3.2.1.1 Cryptococcus sp. S-2 BAA12010.1
BAA12011.1
Q92394   1
 DDB_0214924 (AmyA)   Dictyostelium discoideum AX4 XP_640516.1     1
 α-amylase (Amy;EfAmy)   Euplotes focardii AGU13046.1     1
 ORF   Flammulina velutipes KACC 42777 ADX07284.1     1
 α-amylase (fragment)   Flammulina velutipes L11 AOC97457.1     1
 α-amylase (fragment)   Flammulina velutipes L11 AOC97455.1     1
 α-amylase   Flammulina velutipes L11 AOC97456.1     1
 FFUJ_11362   Fusarium fujikuroi IMI 58289 CCT75344.1     1
 unnamed protein product   Fusarium graminearum PH-1 CEF78721.1     1
 α-amylase 1   Fusarium verticillioides AAZ73168.1 Q3YBZ7   1
 α-amylase (AmyA) 3.2.1.1 Fusicoccum sp. BCC4124 ABG48762.1 Q0Z8K1   1
 ORF   Leptosphaeria maculans JN3 v23.1.3 CBX99032.1 E5A5Y7   1
 LRAMOSA04807   Lichtheimia ramosa CDS12621.1     1
 LRAMOSA09602   Lichtheimia ramosa CDS07079.1     1
 α-amylase (LKA2) 3.2.1.1 Lipomyces kononenkoae SPENCER-MARTINSIAE IGC4052B AAO12212.1 Q8J1E4   1
 α-amylase (Lka1) 3.2.1.1 Lipomyces spencermartinsiae IGC4052B AAC49622.1 Q01117   1
 α-amylase 3.2.1.1 Lipomyces starkeyi KSM 22M AAN75021.1 Q6YF33   1
 MG09640.4   Magnaporthe oryzae 70-15 XP_364795.1 G5EHA8
Q2KHC0
  1
 MG10209.4   Magnaporthe oryzae 70-15 XP_365989.1 Q2KEQ7   1
 MGG_12595   Magnaporthe oryzae 70-15 XP_003720977.1
XP_366696.1
Q2KG13   1
 α-amylase (AmyA;McAmyA) 3.2.1.1 Malbranchea cinnamomea AFD54462.1   3VM7[A] 1
 α-amylase-like (fragment)   Mastigamoeba balamuthi ABD46606.1 Q27ST2   1
 α-amylase (Amy;EcAmy)   Moneuplotes crassus AGU13047.1     1
 NCU09805   Neurospora crassa OR74A EAA34829.1
XP_330455.1
    1
 NCU08131.1   Neurospora crassa OR74A XP_328837.1 Q7S4K0   1
 ORF (fragment)   Nyctotherus ovalis CAI59813.1     1
 α-amylase (AmyI;Amy1) 3.2.1.1 Ophiostoma floccosum ABF72529.1
ABF72530.1
ABF72531.1
Q06SN2   1
 PDE_01201 (Amy13A) 3.2.1.1 Penicillium oxalicum 114-2 EPS26265.1     1
 Pc12g06430   Penicillium rubens Wisconsin 54-1255 CAP80270.1 B6H0D9   1
 Pc16g06140   Penicillium rubens Wisconsin 54-1255 CAP93284.1 B6H8T0   1
 Pc21g10550   Penicillium rubens Wisconsin 54-1255 CAP95952.1 B6HIZ0   1
 Pc16g00630   Penicillium rubens Wisconsin 54-1255 CAP92733.1 B6H6W6   1
 Pc22g12340   Penicillium rubens Wisconsin 54-1255 CAP98522.1 B6HRH6   1
 α-amylase   Penicillium sp. 3-5 AIF73124.1     1
 α-amylase (Amy1) 3.2.1.1 Phanerochaete chrysosporium BKM-F-1767 / ATCC 24725 ABO42285.1 A5Y589   1
 α-amylase (Pnamy3)   Pholiota microspora BAW15173.1     1
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