Glycoside Hydrolase Family 13 / Subf 1

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (274); Uniprot accession (86); PDB accession (9); 3D entries (5); cryst (0)
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 multidomain α-amylase / β-amylase 3.2.1.1
3.2.1.2
Paenibacillus polymyxa AAA85446.1
CAA68344.1
P21543   1
 α-amylase (AmyTV1;AmyE) 3.2.1.1 Thermoactinomyces vulgaris K94 CAA49465.1
CAZ78876.1
Q60051   1
Eukaryota
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 α-amylase (Amyl III) 3.2.1.1 Aspergillus awamori KT-11 BAD06003.1
BAD06005.1
Q76L96   1
 α-amylase (Amyl I) 3.2.1.1 Aspergillus awamori KT-11 BAD06002.1 Q76L99   1
 α-amylase (Amy1;TAA) 3.2.1.1 Aspergillus flavus AAF14264.1
AAS89853.1
AAS89854.1
AAS89855.1
AAS89856.1
AAS89857.1
AAS89858.1
AAS89859.1
AAS89860.1
AAS89861.1
AAS89862.1
AAS89863.1
AAS89864.1
AAS89865.1
AAS89866.1
AAS89867.1
AAS89868.1
AAS89869.1
AAS89870.1
AAS89871.1
AAS89872.1
AAS89873.1
AAS89874.1
AAS89875.1
AAS89876.1
AAS89877.1
AAS89878.1
AAS89879.1
AAS89880.1
AAS89881.1
AAS89882.1
AAS89883.1
AAS89884.1
AAS89885.1
AAS89886.1
AAS89887.1
AAS89888.1
AAS89889.1
AAS89890.1
AAS89891.1
AAS89892.1
Q7LV45
Q96TH4
  1
 α-amylase (AmyA) 3.2.1.1 Aspergillus kawachii IFO 4308 BAD01051.1 Q76CT3   1
 α-amylase (AsaA) 3.2.1.1 Aspergillus kawachii IFO 4308 BAA22993.1
CAX51628.1
O13296   1
 α-amylase (AmyA;AN2018.2) 3.2.1.1 Aspergillus nidulans FGSC A4 EAA64850.1
AAF17103.1
C8VLE6
G5EB45
  1
 α-amylase (AmyB;AN3402.2) 3.2.1.1 Aspergillus nidulans FGSC A4 AAF17100.1
EAA63370.1
C8VHL8
G5EAT0
Q9UV09
  1
 α-amylase (partial) 3.2.1.1 Aspergillus niger   P56271 2AAA[A] 1
 α-amylase A / B (AmyA;AmyB;An05g02100) 3.2.1.1 Aspergillus niger CBS 513.88 CAK44871.1
CAK46324.1
CAL33800.1
A2QL05
A2QL05
2GUY[A]
2GVY[A,B]
1
 acid α-amylase (AamA;An11g03340) 3.2.1.1 Aspergillus niger CBS 513.88 CAL15439.1
A35282
ADX42122.1
CAK48325.1
CAO78410.1
CAX51630.1
A2QW02   1
 cell-wall 4-α-glucanotransferase (AgtA;An09g03100) 2.4.1.25 Aspergillus niger CBS 513.88 CAK40249.1 A2QTS4   1
 4-α-glucanotransferase (AgtB;An12g02460) 2.4.1.25 Aspergillus niger CBS 513.88 CAK41088.1 A2QYT9   1
 α-amylase A (AmyA) 3.2.1.1 Aspergillus niger VAR. AWAMORI UVK143F CAA36966.1 Q02905   1
 α-amylase B (AmyB) 3.2.1.1 Aspergillus niger VAR. AWAMORI UVK143F CAA36967.1
CAL15437.1
CAL15438.1
CAT03384.1
Q02906   1
 Sequence from patent 3.2.1.1 Aspergillus oryzae AAE13949.1
AAE37103.1
AAN27447.1
    1
 α-amylase (AmyB;TAA;Amy1;Amy2;Amy3;Asp o 21;Aspo21) 3.2.1.1 Aspergillus oryzae DSM63303 CAA31218.1
AAA32708.1
ACP34134.1
BAA00336.1
CAA31219.1
CAA31220.1
CAC40348.1
CAT03377.1
CAV35984.1
P0C1B3
P10529
2TAA[A]
3KWX[A]
6TAA[A]
7TAA[A]
1
 α-amylase (Amy1;AmyI;Taα-G1;AO090023000944;AmyA;AmyB) 3.2.1.1 Aspergillus oryzae RIB40 BAE59434.1 P0C1B3 3VX0[A] 1
 α-amylase (Amy2;AmyII;Taα-G2;AO090120000196;AmyA;AmyB) 3.2.1.1 Aspergillus oryzae RIB40 BAE62808.1 P0C1B3   1
 α-amylase (Amy3;AmyIII;Taα-G3;AO090003001210;AmyA) 3.2.1.1 Aspergillus oryzae RIB40 BAA95703.1 P0C1B4   1
 α-amylase (Amy) 3.2.1.1 Aspergillus shirousami BAA01255.1 P30292   1
 α-amylase (AmyA) 3.2.1.1 Aspergillus tubingensis T8.4 AEB80431.1     1
 α-amylase (Amy-CS2) 3.2.1.1 Cryptococcus sp. S-2 BAA12010.1
BAA12011.1
Q92394   1
 α-amylase (AmyA) 3.2.1.1 Fusicoccum sp. BCC4124 ABG48762.1 Q0Z8K1   1
 α-amylase (LKA2) 3.2.1.1 Lipomyces kononenkoae SPENCER-MARTINSIAE IGC4052B AAO12212.1 Q8J1E4   1
 α-amylase (Lka1) 3.2.1.1 Lipomyces spencermartinsiae IGC4052B AAC49622.1 Q01117   1
 α-amylase 3.2.1.1 Lipomyces starkeyi KSM 22M AAN75021.1 Q6YF33   1
 α-amylase (AmyA;McAmyA) 3.2.1.1 Malbranchea cinnamomea AFD54462.1   3VM7[A] 1
 α-amylase (AmyI;Amy1) 3.2.1.1 Ophiostoma floccosum ABF72529.1
ABF72530.1
ABF72531.1
Q06SN2   1
 PDE_01201 (Amy13A) 3.2.1.1 Penicillium oxalicum 114-2 EPS26265.1     1
 α-amylase (Amy1) 3.2.1.1 Phanerochaete chrysosporium BKM-F-1767 / ATCC 24725 ABO42285.1 A5Y589   1
 α-amylase 3.2.1.1 Pichia burtonii 15-1 BAF98616.1 A9ZPM0   1
 α-amylase (RA) 3.2.1.1 Rhizomucor pusillus CAH04003.1
CAX51637.1
    1
 α-amylase (Amy;RpAmy) 3.2.1.1 Rhizomucor pusillus GX-3 AGJ52081.1     1
 α-amylase (Amy;RoAmy) 3.2.1.1 Rhizopus oryzae CICIM F0071 ADL28123.1 E2G4G0   1
 α-amylase (Alp1) 3.2.1.1 Saccharomycopsis fibuligera CAA29233.1
ADU24597.1
P21567   1
 α-amylase (SfAmy KZ) 3.2.1.1 Saccharomycopsis fibuligera KZ ADD80242.1 D4P4Y7   1
 α-amylase (Amy1) 3.2.1.1 Saitozyma flava ABS76467.1 A7LGW4   1
 SPAC4A8.01 (Meu30) 3.2.1.1 Schizosaccharomyces pombe 972h- CAB11471.1
BAB60880.1
CAD62442.2
O14154   1
 cell-wall active enzyme (Aah3;SPCC63.02c) 2.4.1.- Schizosaccharomyces pombe 972h- CAB40006.1
NP_587976.1
Q9Y7S9   1
 α-amylase (Amy1;Amy) 3.2.1.1 Schwanniomyces occidentalis ATCC 26077 CAA34162.1
AAB22383.2
P19269   1
 α-amylase (Swa2) 3.2.1.1 Schwanniomyces occidentalis ATCC 26077 CAA51912.1 Q08806   1
 α-amylase (Amy54;Amy43) 3.2.1.1
3.2.1.116
Sclerotinia sclerotiorum ACN82436.1 C0LW29   1
 α-amylase 3.2.1.1 Thermomyces lanuginosus CAA03110.1
CAA03111.1
    1
 α-amylase (TRa2) 3.2.1.1 Trichoderma viride IAM 5141 / JCM22452 BAG69580.1 B5BQC3   1
 α-amylase 3.2.1.1 Valsaria rubricosa CBS 848.96 CAJ21046.1
CAJ21047.1
    1
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