Glycoside Hydrolase Family 13 / Subf 14

Activities in Familyα-amylase (EC; pullulanase (EC; cyclomaltodextrin glucanotransferase (EC; cyclomaltodextrinase (EC; trehalose-6-phosphate hydrolase (EC; oligo-α-glucosidase (EC; maltogenic amylase (EC; neopullulanase (EC; α-glucosidase (EC; maltotetraose-forming α-amylase (EC; isoamylase (EC; glucodextranase (EC; maltohexaose-forming α-amylase (EC; maltotriose-forming α-amylase (EC; branching enzyme (EC; trehalose synthase (EC; 4-α-glucanotransferase (EC; maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC ; sucrose phosphorylase (EC; malto-oligosyltrehalose trehalohydrolase (EC; isomaltulose synthase (EC; malto-oligosyltrehalose synthase (EC; amylo-α-1,6-glucosidase (EC; α-1,4-glucan: phosphate α-maltosyltransferase (EC; 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (1725); Uniprot accession (230); PDB accession (14); 3D entries (5); cryst (1)
All (1640) Bacteria (1635) Eukaryota (1) unclassified (4) Structure (5 - 1 cryst) Characterized (33)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | ... | 17 |
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 BUW91_09920   Bacillus megaterium JX285 AQU73596.1     14
 BG04_1827 (PulA)   Bacillus megaterium NBRC 15308 = ATCC 14581 AJI23367.1     14
 BG04_4338 (PulA)   Bacillus megaterium NBRC 15308 = ATCC 14581 AJI24033.1     14
 intracellular pullulanase   Bacillus megaterium P6 AWD39017.1     14
 extracellular pullulanase   Bacillus megaterium P6 AWF93940.1     14
 AS52_02034 (PulA)   Bacillus megaterium Q3 AKP76999.1     14
 AS52_04875 (AmyX)   Bacillus megaterium Q3 AKP79828.1     14
 BMQ_2037 (PulA)   Bacillus megaterium QM B1551 ADE69066.1 D5DQD2   14
 BMQ_4837   Bacillus megaterium QM B1551 ADE71839.1 D5DUF0   14
 C0569_09650 (PulA)   Bacillus megaterium STB1 AUO11551.1     14
 C0569_21355 (PulA)   Bacillus megaterium STB1 AUO13737.1     14
 pullulanase, partial   Bacillus megaterium W1210 ANG59290.1     14
 BMWSH_0410 (AmyX)   Bacillus megaterium WSH-002 AEN87294.1     14
 BMWSH_3239 (PulA)   Bacillus megaterium WSH-002 AEN90121.1     14
 C2I28_17995 (PulA)   Bacillus megaterium YC4-R4 AWD68492.1     14
 C2I28_05735 (PulA)   Bacillus megaterium YC4-R4 AWD64622.1     14
 BMMGA3_13410   Bacillus methanolicus MGA3 AIE61072.1     14
 ABE28_011865   Bacillus muralis G25-68 AOH55048.1     14
 DJ92_207 (PulA)   Bacillus mycoides 219298 AIK40141.1     14
 DJ92_1795 (PulA)   Bacillus mycoides 219298 AIK38209.1     14
 BG05_3413 (PulA)   Bacillus mycoides ATCC 6462 AJH20592.1     14
 BG05_1335 (PulA)   Bacillus mycoides ATCC 6462 AJH19071.1     14
 B7492_25100   Bacillus mycoides Gnyt1 ARJ24280.1     14
 B7492_14015   Bacillus mycoides Gnyt1 ARJ22275.1     14
 BcerKBAB4_2416   Bacillus mycoides KBAB4 ABY43627.1 A9VGR4   14
 BcerKBAB4_4533   Bacillus mycoides KBAB4 ABY45689.1 A9VKL2   14
 A361_18985   Bacillus oceanisediminis 2691 AND41148.1     14
 A361_22590   Bacillus oceanisediminis 2691 AND41833.1     14
 BaLi_c32140 (AmyX)   Bacillus paralicheniformis ATCC 9945a AGN37558.1     14
 SC10_B2orf04848   Bacillus paralicheniformis BL-09 AJO19571.1     14
 BLMD_15980   Bacillus paralicheniformis MDJK30 ARA86862.1     14
 Bsel_1254   [Bacillus] selenitireducens MLS10 ADH98766.1 D6XSI0   14
 BS1321_25505   Bacillus simplex NBRC 15720 = DSM 1321 ASS96961.1     14
 BSM4216_2936   Bacillus smithii DSM 4216 AKP48168.1     14
 BS34A_32590 (AmyX)   Bacillus sp. (in: Bacteria) BS34A CEJ78647.1     14
 B1NLA3E_17515   Bacillus sp. 1NLA3E AGK55247.1     14
 CJO35_15660 (PulA)   Bacillus sp. 1s-1 ASV16525.1     14
 BGI23_22790   Bacillus sp. ABP14 AOY18007.1     14
 BGI23_13150   Bacillus sp. ABP14 AOY16119.1     14
 BAQ53_12600   Bacillus sp. B25(2016b) ANP81660.1     14
 BAQ53_22005   Bacillus sp. B25(2016b) ANP83429.1     14
 isoamylase (BsIam) (fragment) Bacillus sp. CICIM 263 AGA03915.1     14
 A6J74_03020   Bacillus sp. FDAARGOS_235 ARC27996.1
 A6J74_21000   Bacillus sp. FDAARGOS_235 ARC31129.1     14
 BGM23_06925   Bacillus sp. FJAT-14266 AOL26313.1     14
 AM500_07530   Bacillus sp. FJAT-18017 ALC89631.1     14
 BSZ43_15550   Bacillus sp. H15-1 APJ28101.1     14
 CU648_28235 (PulA)   Bacillus sp. HBCD-sjtu AUD26106.1     14
 CU648_10175 (PulA) (fragment)   Bacillus sp. HBCD-sjtu AUD26174.1     14
 AZK53_09625   Bacillus sp. IHB B 7164 ANF45933.1     14
 AZK53_22950   Bacillus sp. IHB B 7164 ANF48436.1     14
 MY9_2996   Bacillus sp. JS AFI29529.1     14
 BsLM_2979   Bacillus sp. LM 4-2 AKE24777.1     14
 C1T29_18115 (PulA)   Bacillus sp. MBGLi79 AUZ40065.1     14
 C1T29_17020 (PulA)   Bacillus sp. MBGLi79 AUZ39888.1     14
 CDO84_14160 (PulA)   Bacillus sp. MD-5 ASB62071.1     14
 HW35_06000   Bacillus sp. X1(2014) AIM15928.1     14
 CS527_24210 (PulA)   Bacillus sp. Y-01 AVX10706.1     14
 CS527_10370 (PulA)   Bacillus sp. Y-01 AVX08084.1     14
 QF06_13165   Bacillus sp. YP1 AJO59383.1     14
 BKP58_19085   Bacillus subtilis 29R7-12 API97776.1     14
 BS21228_05430   Bacillus subtilis ATCC 21228 AVL03896.1     14
 BEST7003_2872 (AmyX)   Bacillus subtilis BEST7003 BAM59073.1     14
 C0W65_03160 (PulA)   Bacillus subtilis BJ3-2 AUS11118.1     14
 B7470_04855   Bacillus subtilis Bs-115 ARI85525.1     14
 BS16045_03131   Bacillus subtilis BS16045 AOL98846.1     14
 BSBS38_03000   Bacillus subtilis BS38 AOR99277.1     14
 BS49_32590 (AmyX)   Bacillus subtilis BS49 CEI58225.1     14
 BSn5_05815   Bacillus subtilis BSn5 ADV93791.1     14
 BCV50_18890   Bacillus subtilis CW14 ARV46912.1     14
 CJZ70_05525 (PulA)   Bacillus subtilis DKU_NT_02 ASU97836.1     14
 CJZ71_05600 (PulA)   Bacillus subtilis DKU_NT_03 ASV01710.1     14
 BSK2_14995   Bacillus subtilis GQJK2 ARB38177.1     14
 BSHJ0_03185   Bacillus subtilis HJ0-6 AOA55754.1     14
 AW03_028100 (PulA)   Bacillus subtilis HJ5 AKD36185.1     14
 A4A60_15635   Bacillus subtilis HRBS-10TDI13 AOS69007.1     14
 O7A_16225   Bacillus subtilis KCTC 1028 AKC48575.1     14
 BSR08_11570   Bacillus subtilis KH2 API43107.1     14
 BAX60_20435   Bacillus subtilis MJ01 APH69631.1     14
 B4U62_16110   Bacillus subtilis NCIB 3610 AQZ91883.1     14
 QX56_16225   Bacillus subtilis PS832 AIY98628.1     14
 U712_14865   Bacillus subtilis PY79 AHA78911.1     14
 B657_29930 (AmyX)   Bacillus subtilis QB928 AFQ58838.1     14
 OB04_02971 (AmyX)   Bacillus subtilis SG6 AIX08622.1     14
 CDA59_05595 (PulA)   Bacillus subtilis SR1 ASC81987.1     14
 A1D11_04940   Bacillus subtilis subsp. globigii ATCC 49760 AMR61773.1     14
 AN935_14990   Bacillus subtilis subsp. inaquosorum DE111 AMA53513.1     14
 DKG76_15745 (PulA)   Bacillus subtilis subsp. inaquosorum KCTC 13429 AWM18107.1     14
 BSNT_04363 (AmyX)   Bacillus subtilis subsp. natto BEST195 BAI86501.1 D4G070   14
 AWV81_15255   Bacillus subtilis subsp. natto CGMCC 2108 AMK73399.1     14
 SD85_14665   Bacillus subtilis subsp. spizizenii NRS 231 AJD37420.1     14
 BSUW23_14505 (AmyX)   Bacillus subtilis subsp. spizizenii str. W23 ADM38938.1 E0TXB2   14
 GYO_3241 (PulA)   Bacillus subtilis subsp. spizizenii TU-B-10 AEP87843.1     14
 BFI33_16220   Bacillus subtilis subsp. subtilis 168G AOA12327.1     14
 RP72_16115   Bacillus subtilis subsp. subtilis 3NA AJE95699.1     14
 BSU6051_29930 (AmyX)   Bacillus subtilis subsp. subtilis 6051-HGW AGG62399.1     14
 CXF51_16900 (PulA)   Bacillus subtilis subsp. subtilis BS155 AWX22712.1     14
 AT706_03925   Bacillus subtilis subsp. subtilis BSD-2 ALS81088.1     14
 AWM80_15100   Bacillus subtilis subsp. subtilis CU1050 AMB25250.1     14
 KHRBS_05975   Bacillus subtilis subsp. subtilis D12-5 AMR46103.1     14

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