Glycoside Hydrolase Family 13 / Subf 14

Activities in Familyα-amylase (EC; pullulanase (EC; cyclomaltodextrin glucanotransferase (EC; cyclomaltodextrinase (EC; trehalose-6-phosphate hydrolase (EC; oligo-α-glucosidase (EC; maltogenic amylase (EC; neopullulanase (EC; α-glucosidase (EC; maltotetraose-forming α-amylase (EC; isoamylase (EC; glucodextranase (EC; maltohexaose-forming α-amylase (EC; maltotriose-forming α-amylase (EC; branching enzyme (EC; trehalose synthase (EC; 4-α-glucanotransferase (EC; maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC ; sucrose phosphorylase (EC; malto-oligosyltrehalose trehalohydrolase (EC; isomaltulose synthase (EC; malto-oligosyltrehalose synthase (EC; amylo-α-1,6-glucosidase (EC; α-1,4-glucan: phosphate α-maltosyltransferase (EC; 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (1813); Uniprot accession (230); PDB accession (14); 3D entries (5); cryst (1)
All (1728) Bacteria (1723) Eukaryota (1) unclassified (4) Structure (5 - 1 cryst) Characterized (33)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | ... | 18 |
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 B14_01324 (Amyx_2) (fragment)   Bacillus licheniformis SCDB 14 ARC64325.1     14
 B34_01779 (Amyx_1) (fragment)   Bacillus licheniformis SCDB 34 ARC69219.1     14
 BaDB11_01339 (AmyX)   Bacillus licheniformis SCK B11 ARC60005.1     14
 S100141_02623 (fragment)   Bacillus licheniformis SRCM100141 ARW43942.1     14
 S101441_03152   Bacillus licheniformis SRCM101441 ARW32697.1     14
 glycoside hydrolase (Gh13B)   Bacillus licheniformis SVD1 BAL45510.1     14
 C7M53_16855 (PulA)   Bacillus licheniformis TAB7 AYC52910.1     14
 MUY_003288 (AmyX)   Bacillus licheniformis WX-02 AKQ74420.1     14
 AmyX (fragment)   Bacillus megaterium BP17 AHC08001.1     14
 BMD_4823 (AmyX)   Bacillus megaterium DSM 319 ADF41646.1 D5DN29   14
 BMD_1993 (PulA)   Bacillus megaterium DSM 319 ADF38846.1 D5DE17   14
 BUW91_09920   Bacillus megaterium JX285 AQU73596.1     14
 BG04_1827 (PulA)   Bacillus megaterium NBRC 15308 = ATCC 14581 AJI23367.1     14
 BG04_4338 (PulA)   Bacillus megaterium NBRC 15308 = ATCC 14581 AJI24033.1     14
 OEA_03705 (PulA)   Bacillus megaterium NCT-2 AYE48900.1     14
 OEA_18020 (PulA)   Bacillus megaterium NCT-2 AYE51576.1     14
 intracellular pullulanase   Bacillus megaterium P6 AWD39017.1     14
 extracellular pullulanase   Bacillus megaterium P6 AWF93940.1     14
 AS52_02034 (PulA)   Bacillus megaterium Q3 AKP76999.1     14
 AS52_04875 (AmyX)   Bacillus megaterium Q3 AKP79828.1     14
 BMQ_2037 (PulA)   Bacillus megaterium QM B1551 ADE69066.1 D5DQD2   14
 BMQ_4837   Bacillus megaterium QM B1551 ADE71839.1 D5DUF0   14
 CIB87_22625 (PulA)   Bacillus megaterium SR7 AXI31708.1     14
 CIB87_07815 (PulA)   Bacillus megaterium SR7 AXI32735.1     14
 C0569_09650 (PulA)   Bacillus megaterium STB1 AUO11551.1     14
 C0569_21355 (PulA)   Bacillus megaterium STB1 AUO13737.1     14
 pullulanase, partial   Bacillus megaterium W1210 ANG59290.1     14
 BMWSH_0410 (AmyX)   Bacillus megaterium WSH-002 AEN87294.1     14
 BMWSH_3239 (PulA)   Bacillus megaterium WSH-002 AEN90121.1     14
 C2I28_17995 (PulA)   Bacillus megaterium YC4-R4 AWD68492.1     14
 C2I28_05735 (PulA)   Bacillus megaterium YC4-R4 AWD64622.1     14
 BMMGA3_13410   Bacillus methanolicus MGA3 AIE61072.1     14
 MLA2C4_24455 (PulA)   Bacillus mobilis ML-A2C4 AYF08691.1     14
 MLA2C4_14490 (PulA)   Bacillus mobilis ML-A2C4 AYF06806.1     14
 ABE28_011865   Bacillus muralis G25-68 AOH55048.1     14
 DJ92_207 (PulA)   Bacillus mycoides 219298 AIK40141.1     14
 DJ92_1795 (PulA)   Bacillus mycoides 219298 AIK38209.1     14
 BG05_3413 (PulA)   Bacillus mycoides ATCC 6462 AJH20592.1     14
 BG05_1335 (PulA)   Bacillus mycoides ATCC 6462 AJH19071.1     14
 B7492_25100   Bacillus mycoides Gnyt1 ARJ24280.1     14
 B7492_14015   Bacillus mycoides Gnyt1 ARJ22275.1     14
 BcerKBAB4_2416   Bacillus mycoides KBAB4 ABY43627.1 A9VGR4   14
 BcerKBAB4_4533   Bacillus mycoides KBAB4 ABY45689.1 A9VKL2   14
 A361_18985   Bacillus oceanisediminis 2691 AND41148.1     14
 A361_22590   Bacillus oceanisediminis 2691 AND41833.1     14
 BaLi_c32140 (AmyX)   Bacillus paralicheniformis ATCC 9945a AGN37558.1     14
 SC10_B2orf04848   Bacillus paralicheniformis BL-09 AJO19571.1     14
 BLMD_15980   Bacillus paralicheniformis MDJK30 ARA86862.1     14
 Bsel_1254   [Bacillus] selenitireducens MLS10 ADH98766.1 D6XSI0   14
 BS1321_25505   Bacillus simplex NBRC 15720 = DSM 1321 ASS96961.1     14
 BSM4216_2936   Bacillus smithii DSM 4216 AKP48168.1     14
 BS34A_32590 (AmyX)   Bacillus sp. (in: Bacteria) BS34A CEJ78647.1     14
 B1NLA3E_17515   Bacillus sp. 1NLA3E AGK55247.1     14
 CJO35_15660 (PulA)   Bacillus sp. 1s-1 ASV16525.1     14
 BGI23_22790   Bacillus sp. ABP14 AOY18007.1     14
 BGI23_13150   Bacillus sp. ABP14 AOY16119.1     14
 BAQ53_12600   Bacillus sp. B25(2016b) ANP81660.1     14
 BAQ53_22005   Bacillus sp. B25(2016b) ANP83429.1     14
 isoamylase (BsIam) (fragment) Bacillus sp. CICIM 263 AGA03915.1     14
 DPQ31_13640 (PulA)   Bacillus sp. COPE52 AXK18672.1     14
 DPQ31_24810 (PulA)   Bacillus sp. COPE52 AXK21803.1     14
 DOS87_26415 (PulA)   Bacillus sp. CR71 AXR19397.1     14
 DOS87_15065 (PulA)   Bacillus sp. CR71 AXR17328.1     14
 DS740_16155 (PulA)   Bacillus sp. DM2 AXF34288.1     14
 DPQ26_15070 (PulA)   Bacillus sp. E25 AXR23059.1     14
 DPQ26_26430 (PulA)   Bacillus sp. E25 AXR25129.1     14
 A6J74_03020   Bacillus sp. FDAARGOS_235 ARC27996.1
 A6J74_21000   Bacillus sp. FDAARGOS_235 ARC31129.1     14
 BGM23_06925   Bacillus sp. FJAT-14266 AOL26313.1     14
 AM500_07530   Bacillus sp. FJAT-18017 ALC89631.1     14
 BSZ43_15550   Bacillus sp. H15-1 APJ28101.1     14
 CU648_28235 (PulA)   Bacillus sp. HBCD-sjtu AUD26106.1     14
 CU648_10175 (PulA) (fragment)   Bacillus sp. HBCD-sjtu AUD26174.1     14
 AZK53_09625   Bacillus sp. IHB B 7164 ANF45933.1     14
 AZK53_22950   Bacillus sp. IHB B 7164 ANF48436.1     14
 MY9_2996   Bacillus sp. JS AFI29529.1     14
 BsLM_2979   Bacillus sp. LM 4-2 AKE24777.1     14
 C1T29_18115 (PulA)   Bacillus sp. MBGLi79 AUZ40065.1     14
 C1T29_17020 (PulA)   Bacillus sp. MBGLi79 AUZ39888.1     14
 CDO84_14160 (PulA)   Bacillus sp. MD-5 ASB62071.1     14
 HW35_06000   Bacillus sp. X1(2014) AIM15928.1     14
 CS527_24210 (PulA)   Bacillus sp. Y-01 AVX10706.1     14
 CS527_10370 (PulA)   Bacillus sp. Y-01 AVX08084.1     14
 DOE78_23410 (PulA)   Bacillus sp. Y1 AYA78108.1     14
 DOE78_19315 (PulA)   Bacillus sp. Y1 AYA77418.1     14
 QF06_13165   Bacillus sp. YP1 AJO59383.1     14
 BKP58_19085   Bacillus subtilis 29R7-12 API97776.1     14
 BS21228_05430   Bacillus subtilis ATCC 21228 AVL03896.1     14
 BEST7003_2872 (AmyX)   Bacillus subtilis BEST7003 BAM59073.1     14
 C0W65_03160 (PulA)   Bacillus subtilis BJ3-2 AUS11118.1     14
 B7470_04855   Bacillus subtilis Bs-115 ARI85525.1     14
 BS16045_03131   Bacillus subtilis BS16045 AOL98846.1     14
 BSBS38_03000   Bacillus subtilis BS38 AOR99277.1     14
 BS49_32590 (AmyX)   Bacillus subtilis BS49 CEI58225.1     14
 BSn5_05815   Bacillus subtilis BSn5 ADV93791.1     14
 BCV50_18890   Bacillus subtilis CW14 ARV46912.1     14
 CJZ70_05525 (PulA)   Bacillus subtilis DKU_NT_02 ASU97836.1     14
 CJZ71_05600 (PulA)   Bacillus subtilis DKU_NT_03 ASV01710.1     14
 DTQ03_14845 (PulA)   Bacillus subtilis FB6-3 AXV62563.1     14
 BSK2_14995   Bacillus subtilis GQJK2 ARB38177.1     14

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