Glycoside Hydrolase Family 13 / Subf 14

Activities in Familyα-amylase (EC; pullulanase (EC; cyclomaltodextrin glucanotransferase (EC; cyclomaltodextrinase (EC; trehalose-6-phosphate hydrolase (EC; oligo-α-glucosidase (EC; maltogenic amylase (EC; neopullulanase (EC; α-glucosidase (EC; maltotetraose-forming α-amylase (EC; isoamylase (EC; glucodextranase (EC; maltohexaose-forming α-amylase (EC; maltotriose-forming α-amylase (EC; branching enzyme (EC; trehalose synthase (EC; 4-α-glucanotransferase (EC; maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC ; sucrose phosphorylase (EC; malto-oligosyltrehalose trehalohydrolase (EC; isomaltulose synthase (EC; malto-oligosyltrehalose synthase (EC; amylo-α-1,6-glucosidase (EC; α-1,4-glucan: phosphate α-maltosyltransferase (EC; 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (1750); Uniprot accession (230); PDB accession (14); 3D entries (5); cryst (1)
All (1665) Bacteria (1660) Eukaryota (1) unclassified (4) Structure (5 - 1 cryst) Characterized (33)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | ... | 17 |
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 BTZK2463 (PulA)   Bacillus cereus E33L AAU17796.1
Q63AL5   14
 bcf_23590   Bacillus cereus F837/76 AEW57837.1     14
 bcf_13350   Bacillus cereus F837/76 AEW55792.1     14
 BG03_489 (PulA)   Bacillus cereus FM1 AJG91715.1     14
 BG03_2472 (PulA)   Bacillus cereus FM1 AJG92243.1     14
 FORC21_4604   Bacillus cereus FORC021 AQQ65399.1     14
 FORC21_2560   Bacillus cereus FORC021 AQQ63355.1     14
 FORC60_2623   Bacillus cereus FORC60 AVR32486.1     14
 FORC60_4815   Bacillus cereus FORC60 AVR34626.1     14
 FORC5_4451   Bacillus cereus FORC_005 AKE18988.1     14
 FORC5_2575   Bacillus cereus FORC_005 AKE17112.1     14
 FORC13_2474   Bacillus cereus FORC_013 ALZ61535.1     14
 FORC13_0453   Bacillus cereus FORC_013 ALZ59514.1     14
 FORC24_2515   Bacillus cereus FORC_024 AOM05805.1     14
 FORC24_4508   Bacillus cereus FORC_024 AOM07798.1     14
 FORC47_4630   Bacillus cereus FORC_047 ASL67475.1     14
 FORC47_2610   Bacillus cereus FORC_047 ASL65455.1     14
 FORC48_4729   Bacillus cereus FORC_048 ASI85808.1     14
 FORC48_2665   Bacillus cereus FORC_048 ASI83751.1     14
 BCK_11670   Bacillus cereus FRI-35 AFQ10235.1     14
 BCK_21225   Bacillus cereus FRI-35 AFQ12130.1     14
 BcrFT9_03736   Bacillus cereus FT9 AIE81269.1     14
 BcrFT9_02147   Bacillus cereus FT9 AIE79688.1     14
 AQ16_3290 (PulA)   Bacillus cereus G9241 AJI03839.1     14
 AQ16_5318 (PulA)   Bacillus cereus G9241 AJI05826.1     14
 BCG9842_B0422   Bacillus cereus G9842 ACK93932.1 B7IKW9   14
 BCG9842_B2552   Bacillus cereus G9842 ACK97838.1 B7IJF3   14
 type II pullulanase A (PulA) Bacillus cereus GXBC-3 AFP89962.1     14
 type I pullulanase C (PulC) Bacillus cereus GXBC-3 AFP89964.1     14
 type I pullulanase B (PulB) Bacillus cereus GXBC-3 AFP89963.1     14
 CJ306_25195 (PulA)   Bacillus cereus HBL-AI ASZ68420.1     14
 CJ306_14760 (PulA)   Bacillus cereus HBL-AI ASZ66506.1     14
 WR52_13490   Bacillus cereus HN001 ANC19735.1     14
 WR52_24155   Bacillus cereus HN001 ANC21752.1     14
 BA204_12975   Bacillus cereus K8 ASJ48932.1     14
 BA204_22850   Bacillus cereus K8 ASJ50801.1     14
 BA201_23665   Bacillus cereus M13 ASK16816.1     14
 BA201_13395   Bacillus cereus M13 ASK14853.1     14
 BA202_13110   Bacillus cereus M3 ASI78135.1     14
 BA202_22980   Bacillus cereus M3 ASI79999.1     14
 C1T25_15135 (PulA)   Bacillus cereus MBGJa3 AUZ27497.1     14
 CSW12_23255 (PulA)   Bacillus cereus MLY1 AUB65773.1     14
 CSW12_14060 (PulA)   Bacillus cereus MLY1 AUB64093.1     14
 BCN_4611   Bacillus cereus NC7401 BAL20404.1     14
 BCN_2595   Bacillus cereus NC7401 BAL18388.1     14
 ACN91_04375   Bacillus cereus NJ-W ALC50835.1     14
 ACN91_14385   Bacillus cereus NJ-W ALC52721.1     14
 pullulanase   Bacillus cereus Nws-bc5 AHA37326.1     14
 pullulanase Bacillus cereus OPF-0031 AFJ04510.1     14
 BCQ_2578 (PulA)   Bacillus cereus Q1 ACM13006.1 B9J2W7   14
 BCQ_4515 (AmyX)   Bacillus cereus Q1 ACM14941.1 B9J1A3   14
 BF33_1604 (PulA)   Bacillus cereus S2-8 AJK36167.1     14
 BF33_5074 (PulA)   Bacillus cereus S2-8 AJK34043.1     14
 C2I25_25925 (PulA)   Bacillus cereus TG1-6 AVP48290.1     14
 C2I25_15745 (PulA)   Bacillus cereus TG1-6 AVP46402.1     14
 BC6307_17220   Bacillus cohnii DSM 6307 AST92903.1     14
 CG474_018235 (PulA)   Bacillus cytotoxicus CH_1 AWC62437.1     14
 CG479_017860 (PulA)   Bacillus cytotoxicus CH_13 AWC46196.1     14
 CG480_018705 (PulA)   Bacillus cytotoxicus CH_15 AWC42313.1     14
 CG476_018930 (PulA)   Bacillus cytotoxicus CH_2 AWC58426.1     14
 CG481_018515 (PulA)   Bacillus cytotoxicus CH_23 AWC38223.1     14
 CG482_018665 (PulA)   Bacillus cytotoxicus CH_25 AWC34223.1     14
 CG477_018905 (PulA)   Bacillus cytotoxicus CH_3 AWC54301.1     14
 CG483_018705 (PulA)   Bacillus cytotoxicus CH_38 AWC30176.1     14
 CG475_018940 (PulA)   Bacillus cytotoxicus CH_39 AWC66562.1     14
 CG478_018705 (PulA)   Bacillus cytotoxicus CH_4 AWC50244.1     14
 Bcer98_3365   Bacillus cytotoxicus NVH 391-98 ABS23580.1 A7GTX2   14
 unnamed protein product   Bacillus deramificans CAC60157.1
 amylopullulanase (PulA) (partial) Bacillus deramificans T89.117D AAE10887.1     14
 BEH_24760   Bacillus endophyticus Hbe603 AWG44912.1     14
 BC359_00485   Bacillus flexus KLBMP 4941 AQX56367.1     14
 BC359_03545   Bacillus flexus KLBMP 4941 AQX53454.1     14
 BGM20_08925   Bacillus gibsonii FJAT-10019 AOL30749.1     14
 AM592_10900   Bacillus gobiensis FJAT-4402 ALC82056.1     14
 BH3264 (AmyX)   Bacillus halodurans C-125 BAB06983.1
Q9K7U5   14
 B4U37_17140   Bacillus horikoshii 20a ART77673.1     14
 N288_20045   Bacillus infantis NRRL B-14911 AGX05883.1     14
 CKF48_15920 (PulA)   Bacillus kochii BDGP4 ASV68640.1     14
 BkAM31D_19060 (AmyX)   Bacillus krulwichiae AM31D ARK31767.1     14
 isoamylase (Iam1) (fragment) Bacillus lentus JNU3 AGL34022.1     14
 NCTC4824_03155 (AmyX)   Bacillus lentus NCTC4824 SQI61236.1     14
 BL14DL4_01350   Bacillus licheniformis 14ADL4 AVI46605.1     14
 CPQ91_16045 (PulA)   Bacillus licheniformis ATCC 9789 ATI77277.1     14
 BL1202_03423   Bacillus licheniformis BL1202 AOP16368.1     14
 CD200_15855 (PulA)   Bacillus licheniformis CBA7132 AWV41840.1     14
 BLi03144 or BL00040 (AmyX)   Bacillus licheniformis DSM 13 = ATCC 14580 AAU42005.1
Q65G09   14
 AB684_16090   Bacillus licheniformis HRBL-15TDI7 AMR11631.1     14
 C1T27_16170 (PulA)   Bacillus licheniformis MBGJa67 AUZ31811.1     14
 B37_02746 (Amyx_1) (fragment)   Bacillus licheniformis SCCB 37 ARC74795.1     14
 B14_01323 (Amyx_1) (fragment)   Bacillus licheniformis SCDB 14 ARC64324.1     14
 B14_01324 (Amyx_2) (fragment)   Bacillus licheniformis SCDB 14 ARC64325.1     14
 B34_01779 (Amyx_1) (fragment)   Bacillus licheniformis SCDB 34 ARC69219.1     14
 BaDB11_01339 (AmyX)   Bacillus licheniformis SCK B11 ARC60005.1     14
 S100141_02623 (fragment)   Bacillus licheniformis SRCM100141 ARW43942.1     14
 S101441_03152   Bacillus licheniformis SRCM101441 ARW32697.1     14
 glycoside hydrolase (Gh13B)   Bacillus licheniformis SVD1 BAL45510.1     14
 MUY_003288 (AmyX)   Bacillus licheniformis WX-02 AKQ74420.1     14
 AmyX (fragment)   Bacillus megaterium BP17 AHC08001.1     14
 BMD_4823 (AmyX)   Bacillus megaterium DSM 319 ADF41646.1 D5DN29   14
 BMD_1993 (PulA)   Bacillus megaterium DSM 319 ADF38846.1 D5DE17   14

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