Glycoside Hydrolase Family 13 / Subf 14

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (1454); Uniprot accession (230); PDB accession (7); 3D entries (4); cryst (1)
All (1378) Bacteria (1373) Eukaryota (1) unclassified (4) Structure (4 - 1 cryst) Characterized (33)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | ... | 14 |
Bacteria
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 FORC24_2515   Bacillus cereus FORC_024 AOM05805.1     14
 FORC24_4508   Bacillus cereus FORC_024 AOM07798.1     14
 FORC47_4630   Bacillus cereus FORC_047 ASL67475.1     14
 FORC47_2610   Bacillus cereus FORC_047 ASL65455.1     14
 FORC48_4729   Bacillus cereus FORC_048 ASI85808.1     14
 FORC48_2665   Bacillus cereus FORC_048 ASI83751.1     14
 BCK_11670   Bacillus cereus FRI-35 AFQ10235.1     14
 BCK_21225   Bacillus cereus FRI-35 AFQ12130.1     14
 BcrFT9_03736   Bacillus cereus FT9 AIE81269.1     14
 BcrFT9_02147   Bacillus cereus FT9 AIE79688.1     14
 AQ16_3290 (PulA)   Bacillus cereus G9241 AJI03839.1     14
 AQ16_5318 (PulA)   Bacillus cereus G9241 AJI05826.1     14
 BCG9842_B0422   Bacillus cereus G9842 ACK93932.1 B7IKW9   14
 BCG9842_B2552   Bacillus cereus G9842 ACK97838.1 B7IJF3   14
 type II pullulanase A (PulA) 3.2.1.41 Bacillus cereus GXBC-3 AFP89962.1     14
 type I pullulanase C (PulC) 3.2.1.41 Bacillus cereus GXBC-3 AFP89964.1     14
 type I pullulanase B (PulB) 3.2.1.41 Bacillus cereus GXBC-3 AFP89963.1     14
 CJ306_25195 (PulA)   Bacillus cereus HBL-AI ASZ68420.1     14
 CJ306_14760 (PulA)   Bacillus cereus HBL-AI ASZ66506.1     14
 WR52_13490   Bacillus cereus HN001 ANC19735.1     14
 WR52_24155   Bacillus cereus HN001 ANC21752.1     14
 BA204_12975   Bacillus cereus K8 ASJ48932.1     14
 BA204_22850   Bacillus cereus K8 ASJ50801.1     14
 BA201_23665   Bacillus cereus M13 ASK16816.1     14
 BA201_13395   Bacillus cereus M13 ASK14853.1     14
 BA202_13110   Bacillus cereus M3 ASI78135.1     14
 BA202_22980   Bacillus cereus M3 ASI79999.1     14
 BCN_4611   Bacillus cereus NC7401 BAL20404.1     14
 BCN_2595   Bacillus cereus NC7401 BAL18388.1     14
 ACN91_04375   Bacillus cereus NJ-W ALC50835.1     14
 ACN91_14385   Bacillus cereus NJ-W ALC52721.1     14
 pullulanase   Bacillus cereus Nws-bc5 AHA37326.1     14
 pullulanase 3.2.1.41 Bacillus cereus OPF-0031 AFJ04510.1     14
 BCQ_2578 (PulA)   Bacillus cereus Q1 ACM13006.1 B9J2W7   14
 BCQ_4515 (AmyX)   Bacillus cereus Q1 ACM14941.1 B9J1A3   14
 BF33_1604 (PulA)   Bacillus cereus S2-8 AJK36167.1     14
 BF33_5074 (PulA)   Bacillus cereus S2-8 AJK34043.1     14
 BC6307_17220   Bacillus cohnii DSM 6307 AST92903.1     14
 Bcer98_3365   Bacillus cytotoxicus NVH 391-98 ABS23580.1 A7GTX2   14
 unnamed protein product   Bacillus deramificans CAC60157.1
AAX00708.1
ACK18364.1
CAX87015.1
    14
 amylopullulanase (PulA) (partial) 3.2.1.41 Bacillus deramificans T89.117D AAE10887.1     14
 BC359_00485   Bacillus flexus KLBMP 4941 AQX56367.1     14
 BC359_03545   Bacillus flexus KLBMP 4941 AQX53454.1     14
 BGM20_08925   Bacillus gibsonii FJAT-10019 AOL30749.1     14
 AM592_10900   Bacillus gobiensis FJAT-4402 ALC82056.1     14
 BH3264 (AmyX)   Bacillus halodurans C-125 BAB06983.1
NP_244130.1
Q9K7U5   14
 B4U37_17140   Bacillus horikoshii 20a ART77673.1     14
 N288_20045   Bacillus infantis NRRL B-14911 AGX05883.1     14
 CKF48_15920 (PulA)   Bacillus kochii BDGP4 ASV68640.1     14
 BkAM31D_19060 (AmyX)   Bacillus krulwichiae AM31D ARK31767.1     14
 isoamylase (Iam1) (fragment) 3.2.1.68 Bacillus lentus JNU3 AGL34022.1     14
 BL1202_03423   Bacillus licheniformis BL1202 AOP16368.1     14
 BLi03144 or BL00040 (AmyX)   Bacillus licheniformis DSM 13 = ATCC 14580 AAU42005.1
AAU24646.1
Q65G09   14
 AB684_16090   Bacillus licheniformis HRBL-15TDI7 AMR11631.1     14
 B37_02746 (Amyx_1) (fragment)   Bacillus licheniformis SCCB 37 ARC74795.1     14
 B14_01323 (Amyx_1) (fragment)   Bacillus licheniformis SCDB 14 ARC64324.1     14
 B14_01324 (Amyx_2) (fragment)   Bacillus licheniformis SCDB 14 ARC64325.1     14
 B34_01779 (Amyx_1) (fragment)   Bacillus licheniformis SCDB 34 ARC69219.1     14
 BaDB11_01339 (AmyX)   Bacillus licheniformis SCK B11 ARC60005.1     14
 S100141_02623 (fragment)   Bacillus licheniformis SRCM100141 ARW43942.1     14
 S101441_03152   Bacillus licheniformis SRCM101441 ARW32697.1     14
 glycoside hydrolase (Gh13B)   Bacillus licheniformis SVD1 BAL45510.1     14
 MUY_003288 (AmyX)   Bacillus licheniformis WX-02 AKQ74420.1     14
 AmyX (fragment)   Bacillus megaterium BP17 AHC08001.1     14
 BMD_4823 (AmyX)   Bacillus megaterium DSM 319 ADF41646.1 D5DN29   14
 BMD_1993 (PulA)   Bacillus megaterium DSM 319 ADF38846.1 D5DE17   14
 BUW91_09920   Bacillus megaterium JX285 AQU73596.1     14
 BG04_1827 (PulA)   Bacillus megaterium NBRC 15308 = ATCC 14581 AJI23367.1     14
 BG04_4338 (PulA)   Bacillus megaterium NBRC 15308 = ATCC 14581 AJI24033.1     14
 AS52_02034 (PulA)   Bacillus megaterium Q3 AKP76999.1     14
 AS52_04875 (AmyX)   Bacillus megaterium Q3 AKP79828.1     14
 BMQ_2037 (PulA)   Bacillus megaterium QM B1551 ADE69066.1 D5DQD2   14
 BMQ_4837   Bacillus megaterium QM B1551 ADE71839.1 D5DUF0   14
 pullulanase, partial   Bacillus megaterium W1210 ANG59290.1     14
 BMWSH_0410 (AmyX)   Bacillus megaterium WSH-002 AEN87294.1     14
 BMWSH_3239 (PulA)   Bacillus megaterium WSH-002 AEN90121.1     14
 BMMGA3_13410   Bacillus methanolicus MGA3 AIE61072.1     14
 ABE28_011865   Bacillus muralis G25-68 AOH55048.1     14
 DJ92_207 (PulA)   Bacillus mycoides 219298 AIK40141.1     14
 DJ92_1795 (PulA)   Bacillus mycoides 219298 AIK38209.1     14
 BG05_3413 (PulA)   Bacillus mycoides ATCC 6462 AJH20592.1     14
 BG05_1335 (PulA)   Bacillus mycoides ATCC 6462 AJH19071.1     14
 B7492_25100   Bacillus mycoides Gnyt1 ARJ24280.1     14
 B7492_14015   Bacillus mycoides Gnyt1 ARJ22275.1     14
 A361_18985   Bacillus oceanisediminis 2691 AND41148.1     14
 A361_22590   Bacillus oceanisediminis 2691 AND41833.1     14
 BaLi_c32140 (AmyX)   Bacillus paralicheniformis ATCC 9945a AGN37558.1     14
 SC10_B2orf04848   Bacillus paralicheniformis BL-09 AJO19571.1     14
 BLMD_15980   Bacillus paralicheniformis MDJK30 ARA86862.1     14
 Bsel_1254   [Bacillus] selenitireducens MLS10 ADH98766.1 D6XSI0   14
 BS1321_25505   Bacillus simplex NBRC 15720 = DSM 1321 ASS96961.1     14
 BSM4216_2936   Bacillus smithii DSM 4216 AKP48168.1     14
 B1NLA3E_17515   Bacillus sp. 1NLA3E AGK55247.1     14
 CJO35_15660 (PulA)   Bacillus sp. 1s-1 ASV16525.1     14
 BGI23_22790   Bacillus sp. ABP14 AOY18007.1     14
 BGI23_13150   Bacillus sp. ABP14 AOY16119.1     14
 BAQ53_12600   Bacillus sp. B25(2016b) ANP81660.1     14
 BAQ53_22005   Bacillus sp. B25(2016b) ANP83429.1     14
 BS34A_32590 (AmyX)   Bacillus sp. BS34A CEJ78647.1     14
 isoamylase (BsIam) (fragment) 3.2.1.68 Bacillus sp. CICIM 263 AGA03915.1     14
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