Activities in Sub Family	[retaining] pullulanase (EC 3.2.1.41); Isoamylase (EC 3.2.1.68);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (16); Uniprot accession (17); PDB accession (30); 3D entries (5); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
C3F39_10140		Klebsiella pneumoniae KPNIH48	AUY19104.1		5YN2[A] 2.30 5YN7[A] 2.59 5YNA[A] 1.96 5YNC[A] 2.32 5YND[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.23 5YNE[A] 2.20 5YNH[A] 2.05	13
BB748_23185		Klebsiella pneumoniae Kp_Goe_71070	APM77900.1		6J33[A,B] 1.30 6J34[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.50 6J35[A,B] 1.84 6J4H[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.64	13
pullulanase A (PulA; PUL)	3.2.1.41	Klebsiella pneumoniae UNF5023	CAA36431.1 AAA61976.1 ACH14985.1	P07206	2FGZ[A] 1.75 2FH6[A] α-D-Glcp 1.80 2FH8[A] α-D-Glcp-(1-6)-β-D-Glcp 1.90 2FHB[A] α-D-Glcp-(1-4)-α-D-Glcp 1.80 2FHC[A] α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.85 2FHF[A] α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.65	13
pullulanase (PulA;HMPREF9690_00740)	3.2.1.41	Raoultella ornithinolytica 10-5246	EHT13411.1		2YOC[A,B] 2.88	13
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
limit dextrinase (HvLD99)	3.2.1.41	Hordeum vulgare	AAD04189.1 AAD34347.1 AAD34348.1 AAF98802.1	O48541 Q9FYY0 Q9S7S8	2X4B[A] b-cyclodextrin 2.10 2X4C[A] a-cyclodextrin 2.49 2Y4S[A] b-cyclodextrin 2.10 2Y5E[A] a-cyclodextrin 2.49 4AIO[A] 1.90 4CVW[A,B] 2.67 4J3S[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.75 4J3T[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-D-1-deoxy-Glcp 1.60 4J3U[A,B] 1.70 4J3V[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-6)[α-D-Glcp-(1-4)]-α-D-Glcp6SH-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.45 4J3W[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-6)[α-D-Glcp-(1-4)]-α-D-Glcp-(1-4)-α-D-Glcp 1.67 4J3X[A] α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 1.75	13
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