Glycoside Hydrolase Family 13 / Subf 13

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (817); Uniprot accession (87); PDB accession (19); 3D entries (3); cryst (0)
Bacteria
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 pullulanase A (PulA) 3.2.1.41 Klebsiella pneumoniae UNF5023 CAA36431.1
AAA61976.1
ACH14985.1
P07206
2FGZ[A] 1.75
2FH6[A] α-D-Glcp
1.80
2FH8[A] α-D-Glcp-(1-6)-β-D-Glcp
1.90
2FHB[A] α-D-Glcp-(1-4)-α-D-Glcp
1.80
2FHC[A] α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.85
2FHF[A] α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.65
13
 pullulanase (PulA;HMPREF9690_00740) 3.2.1.41 Raoultella ornithinolytica 10-5246 EHT13411.1  
2YOC[A,B] 2.88
13
Eukaryota
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 limit dextrinase (HvLD99) 3.2.1.41 Hordeum vulgare AAD04189.1
AAD34347.1
AAD34348.1
AAF98802.1
O48541
Q9FYY0
Q9S7S8
2X4B[A] b-cyclodextrin
2.10
2X4C[A] a-cyclodextrin
2.49
2Y4S[A] b-cyclodextrin
2.10
2Y5E[A] a-cyclodextrin
2.49
4AIO[A] 1.90
4CVW[A,B] 2.67
4J3S[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.75
4J3T[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-D-1-deoxy-Glcp
1.60
4J3U[A,B] 1.70
4J3V[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-6)[α-D-Glcp-(1-4)]-α-D-Glcp6SH-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.45
4J3W[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-6)[α-D-Glcp-(1-4)]-α-D-Glcp-(1-4)-α-D-Glcp
1.67
4J3X[A] α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
1.75
13
Top




Last update: 2017-12-01 © Copyright 1998-2017
AFMB - CNRS - Université d'Aix-Marseille