Glycoside Hydrolase Family 13 / Subf 11

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (6077); Uniprot accession (820); PDB accession (7); 3D entries (4); cryst (0)
All (5835) Archaea (46) Bacteria (5713) Eukaryota (70) unclassified (6) Structure (4) Characterized (25)
Archaea
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 isoamylase / 4-α-glucanotransferase (TreX;SSO2094;GlgX;ORF-c06_004;c06021) 2.4.1.25
3.2.1.68
Sulfolobus solfataricus P2 AAK42273.1
CAA69504.1
CAC23738.1
NP_343483.1
P95868
Q7LX99
2VNC[A,B] 3.0
2VR5[A,B] <non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-β-D-Glcp-(1-4)-ASP
α-D-Glcp
2.8
2VUY[A,B] 3.0
11
Bacteria
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 isoamylase (GlgX;b3431) 3.2.1.68 Escherichia coli str. K-12 substr. MG1655 AAC76456.1
AAA58229.1
AAA98735.1
AIZ89569.1
ALI38889.1
AMC96314.1
CAP11380.1
NP_417889.1
P15067
2WSK[A] 2.25
11
 isoamylase (Iam;IsoA) 3.2.1.68 Pseudomonas amyloderamosa SB-15 AAA25854.1
CAA31754.1
P10342
1BF2[A] 2.00
11
Eukaryota
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 isoamylase 1 (Isa1;CrISA1;Sta7) 3.2.1.68 Chlamydomonas reinhardtii CC425 AAP85534.1
AAP88032.1
CAZ78881.1
Q7X8Q2
4J7R[A,B] 2.30
4OKD[A,B] α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-β-D-Glcp-(1-4)-ASP
2.40
11
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