| Activities in Sub Family | [retaining] 4-α-glucanotransferase / amylomaltase (EC 2.4.1.25); α-glucosidase (EC 3.2.1.20); Isoamylase (EC 3.2.1.68); |
|---|---|
| Mechanism | Retaining a |
| Clan | GH-H |
| 3D Structure Status | ( β / α ) 8 barrel |
| Catalytic Nucleophile/Base | Asp (experimental) |
| Catalytic Proton Donor | Glu (experimental) |
| Note | New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431) |
| External resources | CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS; |
| Commercial Enzyme Provider(s) | MEGAZYME; PROZOMIX; |
| Statistics | GenBank accession (51); Uniprot accession (34); PDB accession (12); 3D entries (5); cryst (0) |
| Archaea | ||||||||
| Protein Name | EC# | Reference | Organism | GenBank | Uniprot | PDB/3D | Subf | |
| glycogen debranching enzyme (TreX) | 2.4.1.25 3.2.1.68 |
Saccharolobus shibatae B12 | AAM81590.1 QXJ29377.1 |
Q8NKQ3 | 11 | |||
| isoamylase / 4-α-glucanotransferase (TreX;SSO2094;GlgX;ORF-c06_004;c06021) | 2.4.1.25 3.2.1.68 |
doi doi |
Saccharolobus solfataricus P2 | AAK42273.1 CAA69504.1 CAC23738.1 NP_343483.1 |
P95868 Q7LX99 |
2VNC[A,B] 2VR5[A,B] 2VUY[A,B] 7EAV[A,B] |
11 | |
| isoamylase (TreX) | 3.2.1.68 | Sulfolobus acidocaldarius ATCC33909 | BAA11864.1 | O05152 | 11 | |||
| Bacteria | ||||||||
| Protein Name | EC# | Reference | Organism | GenBank | Uniprot | PDB/3D | Subf | |
| isoamylase (GlgX;Cgl2107;cg2310) | 3.2.1.68 | Corynebacterium glutamicum ATCC 13032 | BAB99500.1 ACC05670.1 ACH05486.1 ACH05487.1 CAC25858.1 CAC25859.1 CAF20443.1 CAK31208.1 CAK31209.1 CCH25246.1 NP_601306.1 |
Q8NNT0 | 11 | |||
| isoamylase (GlgX;b3431) | 3.2.1.68 | pubmed |
Escherichia coli str. K-12 substr. MG1655 | AAC76456.1 AAA58229.1 AAA98735.1 AIZ89569.1 ALI38889.1 AMC96314.1 AUG18134.1 AVI53590.1 AYG17627.1 CAP11380.1 NP_417889.1 |
P15067 | 2WSK[A] | 11 | |
| isoamylase (Iam) | 3.2.1.68 | pubmed |
Flavobacterium sp. | AAB63356.1 | O32611 | 11 | ||
| isoamylase | 3.2.1.68 | pubmed |
Myroides odoratus | BAA82695.1 | Q9RM63 | 11 | ||
| isoamylase (GlgX) | 3.2.1.68 | Pectobacterium carotovorum subsp. carotovorum LY34 | ABV71062.1 ACN29676.1 |
A9XZV3 C0JID0 |
11 | |||
| isoamylase (Iam) | 3.2.1.68 | pubmed |
Pseudomonas sp. | AAA25855.1 AAQ71617.1 AAQ71618.1 CAA00929.1 |
P26501 | 11 | ||
| isoamylase (Iam; IsoA; ISO) | 3.2.1.68 | doi |
Pseudomonas sp. amyloderamosa SB-15 | AAA25854.1 CAA31754.1 |
P10342 | 1BF2[A] | 11 | |
| isoamylase | 3.2.1.68 | Rhodothermus marinus | AAN89211.1 AAE45696.1 AAE45700.1 AAN89215.1 |
11 | ||||
| glycogen debranching enzyme (GlgX; AQF52_6078) | 3.2.1.68 | pubmed |
Streptomyces venezuelae ATCC 15439 | ALO11671.1 | 7U39[A,B,C,D,E,F,G,H,I,J,K,L] 7U3A[A,B,C,D] 7U3B[A,B,C,D,E,F,G,H] 7U3D[A,B,C,D] |
11 | ||
| isoamylase (GlgX;Synpcc7942_0086) | 3.2.1.68 | Synechococcus elongatus PCC 7942 = FACHB-805 | ABB56118.1 | Q31S51 | 11 | |||
| isoamylase (Tfu_1891) | 3.2.1.68 | Thermobifida fusca YX | AAZ55924.1 | Q47NP5 | 11 | |||
| Eukaryota | ||||||||
| Protein Name | EC# | Reference | Organism | GenBank | Uniprot | PDB/3D | Subf | |
| isoamylase-type starch debranching enzyme 3 (Isa3;CrISA3) | 3.2.1.68 | Amaranthus cruentus | BAQ22171.1 | A0A0B6VSY7 | 11 | |||
| At2g39930 (chloroplastic isoamylase 2;AtISA1;Iso1 subunit) | 3.2.1.68 | pubmed |
Arabidopsis thaliana | AAB95278.1 AAN17420.1 AAQ56791.1 AEC09752.1 ANM62439.1 ANM62440.1 NP_181522.1 |
A0A178VW25 A0A1P8B0K2 A0A1P8B0L6 O04196 |
11 | ||
| chloroplastic isoamylase 3 (Isa3;AtISA3;At4g09020) | 3.2.1.68 | Arabidopsis thaliana | CAB78026.1 AAM13879.1 AAM91673.1 AEE82713.1 BAE99109.1 NP_192641.1 |
Q8RWW6 Q9M0S5 |
11 | |||
| isoamylase 1 (Isa1;CrISA1;Sta7) | 3.2.1.68 | Chlamydomonas reinhardtii CC425 | AAP85534.1 AAP88032.1 CAZ78881.1 |
Q7X8Q2 | 4J7R[A,B] 4OKD[A,B] |
11 | ||
| isoamylase | 3.2.1.68 | pubmed |
Oryza sativa | BAA29041.1 | D0TZF0 O80403 |
11 | ||
| isoamylase (Isa1;OsISA1;Su1;Sugary-1;Os08g0520900) | 3.2.1.68 | Oryza sativa Japonica Group | BAC75533.1 ACY56086.1 AII21927.1 BAF24185.1 BAT06299.1 |
D0TZF0 Q84L53 |
11 | |||
| isoamylase 3 (Isa3;TaISA3) | 3.2.1.68 | Triticum aestivum | AEV92948.1 | G9HXG8 | 11 | |||
| isoamylase | 3.2.1.68 | Triticum aestivum | AAL31015.1 AAL31016.1 AAY75324.1 ABA70415.1 ABA70416.1 CAC07514.1 CAC07515.1 CAC41016.2 CAC82925.1 |
Q8VWM3 Q8VWN0 Q8W546 Q8W547 |
11 | |||
| isoamylase (Isa1;Su1;Su-1;Sugary1;Sugary-1) | 3.2.1.68 | Zea mays | ACG43008.1 AAA91298.1 AAB97167.1 AAE25386.1 AAE25387.1 AAR75249.1 ABE20876.1 |
B6U0X5 O22637 Q41742 |
11 | |||
| unclassified | ||||||||
| Protein Name | EC# | Reference | Organism | GenBank | Uniprot | PDB/3D | Subf | |
| amylase | 3.2.1.20 | pubmed |
uncultured organism | ADD61706.1 | D9ZEF2 | 11 | ||
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