Glycoside Hydrolase Family 13 / Subf 11

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (6077); Uniprot accession (820); PDB accession (7); 3D entries (4); cryst (0)
All (5835) Archaea (46) Bacteria (5713) Eukaryota (70) unclassified (6) Structure (4) Characterized (25)
Archaea
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 isoamylase (TreX) 3.2.1.68 Sulfolobus acidocaldarius ATCC33909 BAA11864.1 O05152   11
 glycogen debranching enzyme (TreX) 2.4.1.25
3.2.1.68
Sulfolobus shibatae B12 AAM81590.1 Q8NKQ3   11
 isoamylase / 4-α-glucanotransferase (TreX;SSO2094;GlgX;ORF-c06_004;c06021) 2.4.1.25
3.2.1.68
Sulfolobus solfataricus P2 AAK42273.1
CAA69504.1
CAC23738.1
NP_343483.1
P95868
Q7LX99
2VNC[A,B]
2VR5[A,B]
2VUY[A,B]
11
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 isoamylase (GlgX;Cgl2107;cg2310) 3.2.1.68 Corynebacterium glutamicum ATCC 13032 BAB99500.1
ACC05670.1
ACH05486.1
ACH05487.1
CAC25858.1
CAC25859.1
CAF20443.1
CAK31208.1
CAK31209.1
CCH25246.1
NP_601306.1
Q8NNT0   11
 isoamylase (GlgX;b3431) 3.2.1.68 Escherichia coli str. K-12 substr. MG1655 AAC76456.1
AAA58229.1
AAA98735.1
AIZ89569.1
ALI38889.1
AMC96314.1
CAP11380.1
NP_417889.1
P15067 2WSK[A] 11
 isoamylase (Iam) 3.2.1.68 Flavobacterium sp. AAB63356.1 O32611   11
 isoamylase 3.2.1.68 Myroides odoratus BAA82695.1 Q9RM63   11
 isoamylase (GlgX) 3.2.1.68 Pectobacterium carotovorum subsp. carotovorum LY34 ABV71062.1
ACN29676.1
A9XZV3
C0JID0
  11
 isoamylase (Iam;IsoA) 3.2.1.68 Pseudomonas amyloderamosa SB-15 AAA25854.1
CAA31754.1
P10342 1BF2[A] 11
 isoamylase (Iam) 3.2.1.68 Pseudomonas sp. AAA25855.1
AAQ71617.1
AAQ71618.1
CAA00929.1
P26501   11
 isoamylase 3.2.1.68 Rhodothermus marinus AAN89211.1
AAE45696.1
AAE45700.1
AAN89215.1
    11
 isoamylase (GlgX;Synpcc7942_0086) 3.2.1.68 Synechococcus elongatus PCC 7942 ABB56118.1 Q31S51   11
 isoamylase (Tfu_1891) 3.2.1.68 Thermobifida fusca YX AAZ55924.1 Q47NP5   11
Eukaryota
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 isoamylase-type starch debranching enzyme 3 (Isa3;CrISA3) 3.2.1.68 Amaranthus hybridus subsp. cruentus BAQ22171.1     11
 At1g03310 (chloroplastic isoamylase 2;AtISA2;Iso1 subunit;DBE1) 3.2.1.68 Arabidopsis thaliana AAC72113.1
AAM98123.1
AEE27557.1
AEE27558.1
NP_171830.1
Q8L735
Q9ZVT2
  11
 At2g39930 (chloroplastic isoamylase 2;AtISA1;Iso1 subunit) 3.2.1.68 Arabidopsis thaliana AAB95278.1
AAN17420.1
AAQ56791.1
AEC09752.1
ANM62439.1
ANM62440.1
NP_181522.1
O04196   11
 chloroplastic isoamylase 3 (Isa3;AtISA3;At4g09020) 3.2.1.68 Arabidopsis thaliana CAB78026.1
AAM13879.1
AAM91673.1
AEE82713.1
BAE99109.1
NP_192641.1
Q8RWW6
Q9M0S5
  11
 isoamylase 1 (Isa1;CrISA1;Sta7) 3.2.1.68 Chlamydomonas reinhardtii CC425 AAP85534.1
AAP88032.1
CAZ78881.1
Q7X8Q2 4J7R[A,B]
4OKD[A,B]
11
 isoamylase 1 (fragment) 3.2.1.68 Hordeum vulgare AAD33889.1 Q9XFG6   11
 isoamylase 3.2.1.68 Oryza sativa BAA29041.1 O80403   11
 isoamylase (Isa1;OsISA1;Su1;Sugary-1;Os08g0520900) 3.2.1.68 Oryza sativa Japonica Group BAC75533.1
ACY56086.1
AII21927.1
BAF24185.1
BAT06299.1
Q84L53   11
 isoamylase 3 (Isa3;TaISA3) 3.2.1.68 Triticum aestivum AEV92948.1     11
 isoamylase 3.2.1.68 Triticum aestivum AAL31015.1
AAL31016.1
AAY75324.1
ABA70415.1
ABA70416.1
CAC07514.1
CAC07515.1
CAC41016.2
CAC82925.1
Q8VWM3
Q8VWN0
Q8W546
Q8W547
  11
 isoamylase (Isa1;Su1;Su-1;Sugary1;Sugary-1) 3.2.1.68 Zea mays ACG43008.1
AAA91298.1
AAB97167.1
AAE25386.1
AAE25387.1
AAR75249.1
ABE20876.1
B6U0X5
O22637
Q41742
  11
unclassified
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 amylase 3.2.1.- uncultured organism ADD61706.1 D9ZEF2   11
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