Glycoside Hydrolase Family 103

Activities in Family	peptidoglycan lytic transglycosylase (EC 3.2.1.-)
Mechanism	Retaining
3D Structure Status	lysozyme fold
Catalytic Nucleophile/Base	carbonyl oxygen of C-2 acetamido group of substrate (inferred)
Catalytic Proton Donor	Glu (experimental)
Note	Corresponds to family 3 of the peptidoglycan lytic transglycosylases described by N.T. Blackburn and A.J. Clarke (2001) J. Mol. Evol. 52, 78-84
External resources	CAZypedia;
Statistics	GenBank accession (22029); Uniprot accession (3); PDB accession (13); 3D entries (3); cryst (0)

Bacteria

Protein Name

EC#

Organism

GenBank

Uniprot

PDB/3D

Carbohydrate Ligands

Resolution (Å)

lytic murein transglycosylase B (MltB;b2701;Slt35)

AAB60060.1
AAC75743.1
AIZ90258.1
ALI39582.1
AMC95626.1
AVI52882.1
AYG18309.1
NP_417181.1

1D0K[A]	β-D-GlcpNAc-(1-4)[<non_carb>-(1-3)]-α-D-GlcpNAc β-D-GlcpNAc-(1-4)[<non_carb>-(1-3)]-β-D-GlcpNAc	2.02
1D0L[A]	β-D-GlcpNAc4SO3-(1-1)-<non_carb>	1.97
1D0M[A]	β-D-GlcpNAc-(1-4)-α-D-GlcpNAc β-D-GlcpNAc4SO3-(1-1)-<non_carb>	2.47
1LTM[A]		1.70
1QDR[A]		2.10
1QDT[A]		2.10
1QUS[A]		1.70
1QUT[A]	β-D-GlcpNAc	2.44

soluble lytic transglycosylase (SltB1;PA4001)

4ANR[A]		1.84
5O8X[A,B]		2.50

exo-lytic transglycosylase (SltB3;PA3992)

5ANZ[A]		1.61
5AO7[A]	β-D-GlcpNAc	2.09
5AO8[A]	β-D-GlcpNAc β-D-GlcpNAc-(1-4)[<non_carb>-(1-3)]-β-D-GlcpNAc-(1-1)-methyl	2.09