Carbohydrate-Binding Module Family 50

Activities in FamilyModules of approx. 50 residues found attached to various enzymes from families GH18, GH19, GH23, GH24, GH25 and GH73, i.e. enzymes cleaving either chitin or peptidoglycan. Binding to chitopentaose demonstrated in the case of Pteris ryukyuensis chitinase A [Ohnuma T et al. (2008) J. Biol. Chem. 283:5178-87 (PMID: 18083709)]. CBM50 modules are also found in a multitude of other enzymes targeting the petidoglycan such as peptidases and amidases. These enzymes are not reported in the list below.
NoteAlso known as LysM domains
External resourcesCAZypedia;
Statistics GenBank accession (105207); Uniprot accession (16); PDB accession (37); 3D entries (26); cryst (0)
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 cortex-lytic enzyme SleL   Bacillus cereus ATCC 10876 KFL63465.1  
4S3J[A] 1.60
 cortex-lytic enzyme SleL   Bacillus cereus ATCC 10876 KFL63465.1  
4S3J[A] 1.60
 BSU14040 (YkuD)   Bacillus subtilis subsp. subtilis str. 168 CAA10867.1
CAB13277.1
 
1Y7M[A,B] 2.05
2MTZ[A] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc
NMR
3ZQD[A] NMR
4A1I[A,B,C,D,E,F,G,H] 1.76
4A1J[A,B] 2.20
4A1K[A] 1.75
4A52[A] <carb_like_IM2>
NMR
 N-acetylglucosaminidase (AtlA;EF0799;EF_0799)   Enterococcus faecalis V583 AAO80613.1
AAR60023.1
NP_814543.1
P37710
2MKX[A] NMR
 QR62_09075   Escherichia coli K-12 ALI44012.1  
5FIM[A] NMR
7PVC[A] NMR
 GRF62_14135 (KbP)   Escherichia coli K-12 QHB68149.1  
7VCM[A,B] 1.85
 b0211 (MltD)   Escherichia coli str. K-12 substr. MG1655 AAC73316.1
AAB08633.1
AIZ92633.1
ALI41971.1
AMC97457.1
AUG15008.1
AVI54749.1
AYG20637.1
NP_414747.1
 
1E0G[A] NMR
 spore cortex-specific N-acetylglucosaminidase (SleL;YaaH;BMQ_0021)   Priestia megaterium QM B1551 ADE67156.1
ABS32202.1
YP_003560590.1
 
4S3K[A] 1.70
 spore cortex-specific N-acetylglucosaminidase (SleL;YaaH;BMQ_0021)   Priestia megaterium QM B1551 ADE67156.1
ABS32202.1
YP_003560590.1
 
4S3K[A] 1.70
 D,L-endopeptidase (NlpC/P60;P60_tth;TTHA0266)   Thermus thermophilus HB8 BAD70089.1  
4UZ2[A,B,C,D] 2.50
4UZ3[A,B,C] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-α-D-GlcpNAc
β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc
1.75
4XCM[B] 2.65
 D,L-endopeptidase (NlpC/P60;P60_tth;TTHA0266)   Thermus thermophilus HB8 BAD70089.1  
4UZ2[A,B,C,D] 2.50
4UZ3[A,B] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-α-D-GlcpNAc
β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc
1.75
4XCM[A,B] 2.65
 F0316_17325   Vibrio cholerae RFB16 QEO43340.1  
6U2A[A] 2.30
6UE4[A,B] 2.08
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 chitinase A (EaChiA)   Equisetum arvense BAI22848.1 C7G3I3
5BUM[A,B] 2.50
 LysM effector / extracellular protein 6 (Ecp6)   Fulvia fulva ACF19427.1  
4B8V[A] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-α-D-GlcpNAc
1.59
4B9H[A] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-α-D-GlcpNAc
2.10
 LysM effector / extracellular protein 6 (Ecp6)   Fulvia fulva ACF19427.1  
4B8V[A] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-α-D-GlcpNAc
1.59
4B9H[A] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-α-D-GlcpNAc
2.10
 LysM effector / extracellular protein 6 (Ecp6)   Fulvia fulva ACF19427.1  
4B8V[A] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-α-D-GlcpNAc
1.59
4B9H[A] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-α-D-GlcpNAc
2.10
 LysM type receptor kinase (Lys11)   Lotus japonicus BAI79275.1  
7BAX[A] 2.90
 LysM type receptor kinase (Lys11)   Lotus japonicus BAI79275.1  
7BAX[A] 2.90
 LysM type receptor kinase (Lys11)   Lotus japonicus BAI79275.1  
7BAX[A] 2.90
 chitin elicitor binding protein (CEBiP;OsCEBiP;Os03g0133400)   Oryza sativa Japonica Group BAF10789.1
AAN05509.1
ABF93833.1
BAE95828.1
BAG93257.1
BAS82158.1
 
5JCD[A,B,C] 2.40
5JCE[A,B] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc
2.51
 chitin elicitor binding protein (CEBiP;OsCEBiP;Os03g0133400)   Oryza sativa Japonica Group BAF10789.1
AAN05509.1
ABF93833.1
BAE95828.1
BAG93257.1
BAS82158.1
 
5JCD[A,B,C] 2.40
5JCE[A,B] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc
2.51
 chitinase A (ChiA;PrChiA)   Pteris ryukyuensis BAE98134.1 Q0WYK2
4PXV[A,B,C,D] 1.80
5YLG[A,B,C,D] 1.48
 lectin (MGG_03307)   Pyricularia oryzae 70-15 EDK00177.1  
2L9Y[A] NMR
5C8O[A] 2.09
5C8P[A] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc
2.20
5C8Q[B] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc
1.90
 chitinase/lysozyme (VcLysM1;VcLysM2)   Volvox carteri f. nagariensis AAC13727.1  
5YZ6[A] NMR
 chitinase/lysozyme (VcLysM1;VcLysM2)   Volvox carteri f. nagariensis AAC13727.1  
5K2L[A] 1.20
5YZK[A] NMR
 LysM effector (MYCGRDRAFT_105487)   Zymoseptoria tritici IPO323 XP_003850050.1  
6Q40[A,B,C,D] β-D-GlcpNAc-(1-4)-β-D-GlcpNAc-(1-4)-β-D-GlcpNAc
2.41
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