Auxiliary Activity Family 9

Activities in FamilyAA9 (formerly GH61) proteins are copper-dependent lytic polysaccharide monooxygenases (LPMOs); cleavage of cellulose chains with oxidation of carbons C1 and/or C4 and C-6); lytic cellulose monooxygenase (C1-hydroxylating) (EC 1.14.99.54); lytic cellulose monooxygenase (C4-dehydrogenating) (EC 1.14.99.56)
Mechanism monooxygenase
NoteAA9 (formerly GH61). The enzymes in this family were originally classified as a glycoside hydrolases (GH61) based on very weak endo-1,4-b-D-glucanase activity in one family member. They are now reclassified in the AA category of CAZy. Because a significant literature is associated with the old name GH61, we recommend to describe these enzymes as "AA9 (formerly GH61)".
External resourcesCAZypedia;
Statistics GenBank accession (1046); Uniprot accession (36); PDB accession (56); 3D entries (20); cryst (0)
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 lytic polysaccharide monooxygenase (CvAA9A) 1.14.99.54
1.14.99.56
Achaetomiella virescens CBS 547.75 AST24379.1 A0A223GEC9
5NLT[A,B,C,D,E,F] 1.90
6YDC[A,B,C,D] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
2.00
6YDD[A,B] β-D-Glcp-(1-4)-β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
2.80
6YDE[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
2.20
6YDF[A,B] 2.12
 NFIA_012990   Aspergillus fischeri NRRL 181 EAW22610.1  
7OVA[A]
 lytic cellulose monooxygenase (AfAA9_B; AFUA_4G07850; Afu4g07850) 1.14.99.54
1.14.99.56
Aspergillus fumigatus Af293 XP_752040.1
EAL90002.1
 
5X6A[A] 1.70
6H1Z[A,B] 1.57
6HA5[A,B] 1.87
6HAQ[A,B] 1.37
 lytic cellulose monooxygenase (CsLPMO9;CERSUDRAFT_85414) 1.14.99.54 Gelatoporia subvermispora B EMD35432.1  
7EXK[A]
  lytic cellulose monooxygenase (C1-hydroxylating) (HETIRDRAFT_166613; HiLPMO9B) (HiLPMO9B) 1.14.99.54 Heterobasidion irregulare TC 32-1 ETW87087.1 W4KMP1
5NNS[A,B] 2.10
 lytic cellulose monooxygenase (C1-hydroxylating / C4-dehydrogenating) (LsAA9A) 1.14.99.54
1.14.99.56
Lentinus similis ALN96977.1 A0A0S2GKZ1
5ACF[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.80
5ACG[A] 1.91
5ACH[A] 1.28
5ACI[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.75
5ACJ[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.70
5N04[A] 1.76
5N05[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.58
5NKW[A] β-D-Glcp-(1-4)-β-D-Manp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Manp-(1-4)-β-D-Glcp-(1-4)-β-D-Manp
β-D-Glcp-(1-4)-β-D-Manp-(1-4)-β-D-Manp
1.48
5NLN[A] β-D-Xylp
β-D-Xylp-(1-1)-<non_carb>
1.90
5NLO[A] β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp
1.33
5NLP[A] β-D-Xylp
β-D-Xylp-(1-4)-α-D-Xylp
β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp
1.59
5NLQ[A] β-D-Xylp
β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-α-D-Xylp
β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp
1.50
5NLR[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
2.00
5NLS[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.75
6YDG[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
1.90
7NIM[A] D-1,3,4-deoxy-Ribp
1.45
7NIN[A] D-1,3,4-deoxy-Ribp
1.40
7PTZ[A]
 ORF   Lentinus similis QEM23792.1  
6RS6[A] 1.60
6RS7[A] 1.60
6RS8[A] 1.58
6RS9[A] β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp-(1-4)-β-D-Xylp
1.40
 LPMO9F   Malbranchea cinnamomea FCH 10.5 QDV60870.1  
7NTL[A] 1.38
 Lytic cellulose monooxygenase (C4-dehydrogenating) (PMO-2;NcPMO-2;NcLPMO9D;GH61-4;NCU01050) (LPMO9D) 1.14.99.56 Neurospora crassa OR74A CAD21296.1
EAA32426.1
XP_326543.1
Q1K8B6
Q8WZQ2
4EIR[A,B] 1.10
5TKF[A,B,C,D] 2.10
5TKG[A,B] 1.20
5TKH[A,B] 1.20
5TKI[A,B] 1.50
  lytic cellulose monooxygenase (C1-hydroxylating) 9F (PMO-03328;LPMO-03328;NcLPMO9F;GH61-6;NCU03328) (LPMO9F) 1.14.99.54 Neurospora crassa OR74A CAD70347.1
EAA26656.1
XP_322586.1
Q1K4Q1
Q873G1
4QI8[A,B] 1.10
 lytic cellulose polysaccharide monooxygenase (C1-hydroxylating/C4-dehydrogenating) (PMO-3;NcLPMO9M;GH61-13;NcPMO-3;NCU07898) (LPMO9M) 1.14.99.54
1.14.99.56
Neurospora crassa OR74A EAA33178.1
XP_328604.1
Q7SA19
4EIS[A,B] 1.37
  Lytic cellulose monooxygenase (C4-dehydrogenating) (PMO-02916;LPMO-02916;NcLPMO9C;GH61-3;NCU02916) (LPMO9C) 1.14.99.56 Neurospora crassa OR74A EAA36362.1
XP_330104.1
Q7SHI8
4D7U[A,B] 1.56
4D7V[A,B] 1.90
 Lytic cellulose monooxygenase (C4-dehydrogenating) (GH61-1;NcLPMO9A;NCU02240) 1.14.99.56 Neurospora crassa OR74A EAA30263.1
XP_331016.1
Q7S439
5FOH[A] 1.60
 lytic cellulose monooxygenase (C1-hydroxylating) (Gh61D;PcGH61D;PcLPMO9D) 1.14.99.54 Phanerochaete chrysosporium K-3 BAL43430.1 H1AE14
4B5Q[A,B] 1.75
 lytic polysaccharide monooxygenase (Lpmo1)   Talaromyces verruculosus PV2007 QBL14594.1  
7A8V[A] α-D-Manp
1.95
 lytic cellulose monooxygenase (C1-hydroxylating) (TaAA9;TaAA9A;TaGH61;TaGH61A;TaLPMO9A) 1.14.99.54 Thermoascus aurantiacus ACS05720.1
ABW56451.1
G3XAP7
2YET[A,B] 1.50
3ZUD[A] 1.25
7PU1[A]
 lytic cellulose monooxygenase (C1-hydroxylating) (MtPMO3;MYCTH_92668) 1.14.99.54 Thermothelomyces thermophilus ATCC 42464 AEO56665.1 G2QAB5
5UFV[A,B,C,D,E,F] 2.45
 lytic cellulose monooxygenase (C1-hydroxylating) (THITE_2122979) 1.14.99.54 Thermothielavioides terrestris NRRL 8126 XP_003657366.1
ACE10234.1
AEO71030.1
D0VWZ9
G2RGE5
3EII[A,B,C,D] 2.25
3EJA[A,B,C,D] 1.90
 lytic polysaccharide mono-oxygenase active on cellulose (EG7;HjGH61B) (Cel61B=GH61B)   Trichoderma reesei QM6A AAP57753.1
ABH82048.1
ACK19226.1
ACR92640.1
 
2VTC[A,B] 1.6
 lytic cellulose monooxygenase (C1-hydroxylating/C4-dehydrogenating) (EGIV;Egl4;EG4) (formerly endo-β-1,4-glucanase IV) (LPMO9A) 1.14.99.54
1.14.99.56
Trichoderma reesei RUTC-30 CAA71999.1 G0R6T8
O14405
5O2W[A] 2.00
5O2X[A] 0.95
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